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Literature summary extracted from

  • Huang, X.; Yan, W.; Gao, D.; Tong, M.; Tai, H.H.; Zhan, C.G.
    Structural and functional characterization of human microsomal prostaglandin E synthase-1 by computational modeling and site-directed mutagenesis (2006), Bioorg. Med. Chem., 14, 3553-3562.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 from human placenta cDNA library, expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.3 three-dimensional model of substrate-domain and its binding with subsrtates prostaglandin H2 and glutathione. Residue Y130 plays a key role in binding with prostaglandin H2 and in the catalytic process. R110 and T114 interact intensively with the carbonyl tail of prostaglandin H2, whereas Q36 and Q134 only enhance the prostaglandin H2 binding affinity. Prostaglandin H2 interacts with glutathione through hydrogen binding between the peroxy group of prostaglandin H2 and the -SH group of glutathione Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.3 Q134E fivefold increase in Km value Homo sapiens
5.3.99.3 Q36E tenfold increase in Km value Homo sapiens
5.3.99.3 R110T 18% of wild-type activity Homo sapiens
5.3.99.3 T114V 21% of wild-type activity Homo sapiens
5.3.99.3 Y130I loss of most of the enzymic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.99.3 0.13
-
 (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate wild-type, 0°C, pH 7.2 Homo sapiens
5.3.99.3 0.734
-
 (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate mutant Q134E, 0°C, pH 7.2 Homo sapiens
5.3.99.3 1.61
-
 (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate mutant Q36E, 0°C, pH 7.2 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 membrane
-
 Homo sapiens 16020
-
5.3.99.3 microsome
-
 Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 (5Z,13E)-(15S)-9a,11a-epidioxy-15-hydroxyprosta-5,13-dienoate + GSH
-
 Homo sapiens (5Z,13E)-(15S)-11a,15-dihydroxyprosta-5,13-dienoate + GSSG
-
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