Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toyota, E.; Iyaguchi, D.; Sekizaki, H.; Itoh, K.; Tanizawa, K.
    Kinetic properties of three isoforms of trypsin isolated from the pyloric caeca of chum salmon (Oncorhynchus keta) (2007), Biol. Pharm. Bull., 30, 1648-1652.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.01
-
 Tos-L-Arg-OMe at 25°C Bos taurus
3.4.21.4 0.01
-
 Tos-L-Arg-OMe at 5°C Bos taurus
3.4.21.4 0.01
-
 Tos-L-Arg-OMe at 15°C Bos taurus
3.4.21.4 0.02
-
 Tos-L-Arg-OMe at 35°C Bos taurus
3.4.21.4 0.02
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 5°C Oncorhynchus keta
3.4.21.4 0.02
-
 Tos-L-Arg-OMe isozyme ST-1, at 5°C Oncorhynchus keta
3.4.21.4 0.02
-
 Tos-L-Arg-OMe isozyme ST-2, at 15°C Oncorhynchus keta
3.4.21.4 0.02
-
 Tos-L-Arg-OMe isozyme ST-2, at 25°C Oncorhynchus keta
3.4.21.4 0.02
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 5°C Oncorhynchus keta
3.4.21.4 0.02
-
 Tos-L-Arg-OMe isozyme ST-2, at 5°C Oncorhynchus keta
3.4.21.4 0.02
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 15°C Oncorhynchus keta
3.4.21.4 0.02
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 25°C Oncorhynchus keta
3.4.21.4 0.02
-
 Tos-L-Arg-OMe isozyme ST-3, at 25°C Oncorhynchus keta
3.4.21.4 0.02
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 5°C Oncorhynchus keta
3.4.21.4 0.02
-
 Tos-L-Arg-OMe isozyme ST-3, at 5°C Oncorhynchus keta
3.4.21.4 0.03
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 15°C Oncorhynchus keta
3.4.21.4 0.03
-
 Tos-L-Arg-OMe isozyme ST-1, at 15°C Oncorhynchus keta
3.4.21.4 0.03
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 25°C Oncorhynchus keta
3.4.21.4 0.03
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 15°C Oncorhynchus keta
3.4.21.4 0.03
-
 Tos-L-Arg-OMe isozyme ST-2, at 35°C Oncorhynchus keta
3.4.21.4 0.03
-
 Tos-L-Arg-OMe isozyme ST-3, at 15°C Oncorhynchus keta
3.4.21.4 0.04
-
 Tos-L-Arg-OMe isozyme ST-1, at 25°C Oncorhynchus keta
3.4.21.4 0.04
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 25°C Oncorhynchus keta
3.4.21.4 0.04
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 35°C Oncorhynchus keta
3.4.21.4 0.04
-
 Tos-L-Arg-OMe isozyme ST-3, at 35°C Oncorhynchus keta
3.4.21.4 0.05
-
 Tos-L-Arg-OMe isozyme ST-1, at 35°C Oncorhynchus keta
3.4.21.4 0.05
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 35°C Oncorhynchus keta
3.4.21.4 0.07
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 35°C Oncorhynchus keta
3.4.21.4 0.2
-
 benzoyl-L-arginine-p-nitroanilide at 5°C Bos taurus
3.4.21.4 0.26
-
 benzoyl-L-arginine-p-nitroanilide at 25°C Bos taurus
3.4.21.4 0.27
-
 benzoyl-L-arginine-p-nitroanilide at 15°C Bos taurus
3.4.21.4 0.44
-
 benzoyl-L-arginine-p-nitroanilide at 35°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 24000
-
 SDS-PAGE Oncorhynchus keta

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Bos taurus
-
-
-
3.4.21.4 Oncorhynchus keta
-
 chum salmon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 ammonium sulfate fractionation, acetone precipitation, benzamidine-Sepharose 6B affinity column chromatography, Sephadex G-75 gel filtration, and DEAE-Sepharose column chromatography Oncorhynchus keta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 pyloric cecum
-
 Oncorhynchus keta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 0.23
-
 clarified extract, at 25°C Oncorhynchus keta
3.4.21.4 5.56
-
 isozyme ST-3, after 24.2fold purification, at 25°C Oncorhynchus keta
3.4.21.4 6.23
-
 isozyme ST-2, after 27.1fold purification, at 25°C Oncorhynchus keta
3.4.21.4 6.43
-
 isozyme ST-1, after 28fold purification, at 25°C Oncorhynchus keta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 benzoyl-L-arginine-p-nitroanilide + H2O
-
 Bos taurus benzoyl-L-arginine + p-nitroaniline
-
 ?
3.4.21.4 benzoyl-L-arginine-p-nitroanilide + H2O
-
 Oncorhynchus keta benzoyl-L-arginine + p-nitroaniline
-
 ?
3.4.21.4 Tos-L-Arg-OMe + H2O
-
 Bos taurus ?
-
 ?
3.4.21.4 Tos-L-Arg-OMe + H2O
-
 Oncorhynchus keta ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 ST-1 isozyme Oncorhynchus keta
3.4.21.4 ST-2 isozyme Oncorhynchus keta
3.4.21.4 ST-3 isozyme Oncorhynchus keta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 0.3
-
 benzoyl-L-arginine-p-nitroanilide at 5°C Bos taurus
3.4.21.4 0.64
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 5°C Oncorhynchus keta
3.4.21.4 0.76
-
 benzoyl-L-arginine-p-nitroanilide at 15°C Bos taurus
3.4.21.4 0.83
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 5°C Oncorhynchus keta
3.4.21.4 0.94
-
 benzoyl-L-arginine-p-nitroanilide at 25°C Bos taurus
3.4.21.4 1.06
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 5°C Oncorhynchus keta
3.4.21.4 1.27
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 15°C Oncorhynchus keta
3.4.21.4 2
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 25°C Oncorhynchus keta
3.4.21.4 2.43
-
 benzoyl-L-arginine-p-nitroanilide at 35°C Bos taurus
3.4.21.4 2.72
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 15°C Oncorhynchus keta
3.4.21.4 2.74
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 15°C Oncorhynchus keta
3.4.21.4 3 6 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 15°C Oncorhynchus keta
3.4.21.4 3.13
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 25°C Oncorhynchus keta
3.4.21.4 3.86
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-3, at 35°C Oncorhynchus keta
3.4.21.4 4.06
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 25°C Oncorhynchus keta
3.4.21.4 5.54
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 25°C Oncorhynchus keta
3.4.21.4 6.74
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-2, at 35°C Oncorhynchus keta
3.4.21.4 7.7
-
 benzoyl-L-arginine-p-nitroanilide isozyme ST-1, at 35°C Oncorhynchus keta
3.4.21.4 41.3
-
 Tos-L-Arg-OMe isozyme ST-1, at 5°C Oncorhynchus keta
3.4.21.4 46.2
-
 Tos-L-Arg-OMe at 5°C Bos taurus
3.4.21.4 61.7
-
 Tos-L-Arg-OMe isozyme ST-2, at 5°C Oncorhynchus keta
3.4.21.4 64.5
-
 Tos-L-Arg-OMe isozyme ST-1, at 15°C Oncorhynchus keta
3.4.21.4 68.4
-
 Tos-L-Arg-OMe isozyme ST-3, at 5°C Oncorhynchus keta
3.4.21.4 69.2
-
 Tos-L-Arg-OMe at 15°C Bos taurus
3.4.21.4 94.3
-
 Tos-L-Arg-OMe isozyme ST-1, at 25°C Oncorhynchus keta
3.4.21.4 94.3
-
 Tos-L-Arg-OMe isozyme ST-1, at 35°C Oncorhynchus keta
3.4.21.4 105
-
 Tos-L-Arg-OMe isozyme ST-2, at 15°C Oncorhynchus keta
3.4.21.4 109
-
 Tos-L-Arg-OMe at 25°C Bos taurus
3.4.21.4 139
-
 Tos-L-Arg-OMe at 35°C Bos taurus
3.4.21.4 154
-
 Tos-L-Arg-OMe isozyme ST-3, at 15°C Oncorhynchus keta
3.4.21.4 159
-
 Tos-L-Arg-OMe isozyme ST-3, at 25°C Oncorhynchus keta
3.4.21.4 189
-
 Tos-L-Arg-OMe isozyme ST-3, at 35°C Oncorhynchus keta
3.4.21.4 200
-
 Tos-L-Arg-OMe isozyme ST-2, at 35°C Oncorhynchus keta
3.4.21.4 234
-
 Tos-L-Arg-OMe isozyme ST-2, at 25°C Oncorhynchus keta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.4 Oncorhynchus keta isozyme ST-2, isoelectric focusing
-
 5.4
3.4.21.4 Oncorhynchus keta isozyme ST-3, isoelectric focusing
-
 5.6
3.4.21.4 Oncorhynchus keta isozyme ST-1, isoelectric focusing
-
 5.8