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Literature summary extracted from

  • Anish, R.; Rao, M.
    Biochemical characterization of a novel beta-1-3, 1-4 glucan 4-glucanohydrolase from Thermomonospora sp. having a single active site for lichenan and xylan (2007), Biochimie, 89, 1489-1497.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 2,4,6-Trinitrobenzenesulfonic acid
-
 Thermomonospora sp.
3.2.1.73 5,5'-dithiobis 2-nitrobenzoic acid
-
 Thermomonospora sp.
3.2.1.73 o-phthaldehyde
-
 Thermomonospora sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 64120
-
 gel filtration Thermomonospora sp.
3.2.1.73 64240
-
 calculated from amino acid sequence Thermomonospora sp.
3.2.1.73 64500
-
 SDS-PAGE Thermomonospora sp.
3.2.1.73 66730
-
 MALDI-TOF mass spectrometry Thermomonospora sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Thermomonospora sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 DEAE Sephadex A50 column chromatography and Sephacryl S-200 gel filtration Thermomonospora sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 18.89
-
 enzyme from culture filtrate, using xylan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.
3.2.1.73 25
-
 enzyme from culture filtrate, using lichenan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.
3.2.1.73 598.6
-
 after 31.6fold purification, using xylan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.
3.2.1.73 792.2
-
 after 31.6fold purification, using lichenan as a substrate, at 50°C, pH 7.0 Thermomonospora sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 lichenan + H2O
-
 Thermomonospora sp. D-glucose + ?
-
 ?
3.2.1.73 additional information no activity towards avicel, laminarin, gum arabic, p-nitrophenyl glucoside, and p-nitrophenyl xylopyranoside Thermomonospora sp. ?
-
 ?
3.2.1.73 xylan + H2O
-
 Thermomonospora sp. xylose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1-3, 1-4 glucan 4-glucanohydrolase
-
 Thermomonospora sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 50
-
-
 Thermomonospora sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 80
-
 half-life of 5 min at 80°C Thermomonospora sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 7
-
-
 Thermomonospora sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 4 8
-
 Thermomonospora sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.73 Thermomonospora sp. isoelectric focusing
-
 4.27