Literature summary extracted from

Antoine, T.; Bosso, C.; Heyraud, A.; Samain, E.
Large scale in vivo synthesis of globotriose and globotetraose by high cell density culture of metabolically engineered Escherichia coli (2005), Biochimie, 87, 197-203.

Application

EC Number	Application	Comment	Organism
2.4.1.79	synthesis	synthesis of globotetraose (GalNAcb-3Gala-4Galb-4Glc)by the high cell density culture of an Escherichia coli strain over-expressing the Neisseria meningitidis lgtC gene for alpha-1,4-Gal transferase. The strain which is devoid of both alpha and beta galactosidase activity is fed with glycerol as the energy and carbon source and with lactose as precursor for globotriose synthesis. After complete exhaustion of lactose, globotriose could serve as an alternative acceptor for LgtC and the formation of a series of polygalactosylated compounds is observed. The system is extended to the synthesis of globotetraose (GalNAcbeta-3Gala-4Galbeta-4Glc) by overexpressing two additional genes: lgtD from Haemophilus influenzae Rd which encodes a beta-1,3-GalNAc transferase and wbpP from Pseudomonas aeruginosa which encodes a UDP-GalNAc C4 epimerase. Globotetraose could also be produced from exogenous globotriose which is actively taken up by the cells	Haemophilus influenzae

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.79	Haemophilus influenzae	-	-	-
2.4.1.79	Haemophilus influenzae RD	-	-	-

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Release 2023.1 (January 2023)