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Literature summary extracted from

  • Landman, O.E.
    Properties and induction of beta-galactosidase in Bacillus megaterium (1957), Biochim. Biophys. Acta, 23, 558-569.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 D-fructose slightly stimulating Priestia megaterium
3.2.1.23 D-glucose slightly stimulating Priestia megaterium
3.2.1.23 maltose slightly stimulating Priestia megaterium
3.2.1.23 additional information no activation by melibiose and raffinose Priestia megaterium
3.2.1.23 sucrose slightly stimulating Priestia megaterium

General Stability

EC Number General Stability Organism
3.2.1.23 non-specific enzyme stabilization by amino acids, overview Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Cs+ stimulates Priestia megaterium
3.2.1.23 K+ stimulates Priestia megaterium
3.2.1.23 Li+ stimulates Priestia megaterium
3.2.1.23 Mn2+ stimulates Priestia megaterium
3.2.1.23 Na+ stimulates Priestia megaterium
3.2.1.23 NH4+ stimulates Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Priestia megaterium
-
 inducible enzyme
-
3.2.1.23 Priestia megaterium KM / ATCC 13632
-
 inducible enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 native enzyme by MnSO4 or streptomycin precipitation, ammonium sulfate fractionation, and starch column electrophoresis Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
3.2.1.23 -20°C, crude enzyme extract, 24 h, fully stable, loss of 49% activity after 12 days, partially purified enzyme is stable for 60 days Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Priestia megaterium 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Priestia megaterium KM / ATCC 13632 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Priestia megaterium beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Priestia megaterium KM / ATCC 13632 beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.23 additional information the enzyme hydrolyzes methyl-, butyl-, and phenyl-beta-D-galactosides, no activity with thiomethyl-and thiophenyl-beta-D-galactosides or with methyl-beta-L-arabinoside Priestia megaterium ?
-
 ?
3.2.1.23 additional information the enzyme hydrolyzes methyl-, butyl-, and phenyl-beta-D-galactosides, no activity with thiomethyl-and thiophenyl-beta-D-galactosides or with methyl-beta-L-arabinoside Priestia megaterium KM / ATCC 13632 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 40
-
 assay at Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 55
-
 5 min, loss of 42% activity in 0.5 M K2HPO4 buffer, no activity loss in 1.5 M K2HP04 Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7.3 8
-
 Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 6 9 pH profile Priestia megaterium