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Literature summary extracted from
- Seprase: An overview of an important matrix serine protease (2008), Biochim. Biophys. Acta, 1784, 1130-1145.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.B28 | - |
Mus musculus |
| 3.4.21.B28 | expressed in WI38 cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.B28 | 4-(2-aminoethyl)-benzenesulfonylfluoride | 5 mM | Homo sapiens |  |
| 3.4.21.B28 | 4-(2-aminoethyl)-benzenesulfonylfluoride | 5 mM | Mus musculus | |
| 3.4.21.B28 | 4-amidino-phenylsulfonylfluoride | 0.5 mM | Homo sapiens | |
| 3.4.21.B28 | 4-amidino-phenylsulfonylfluoride | 0.5 mM | Mus musculus | |
| 3.4.21.B28 | diisopropyl fluorophosphate | 0.005 mM | Homo sapiens | |
| 3.4.21.B28 | diisopropylfluorophosphate | 0.005 mM | Mus musculus | |
| 3.4.21.B28 | HgCl2 | - |
Homo sapiens | |
| 3.4.21.B28 | HgCl2 | - |
Mus musculus | |
| 3.4.21.B28 | N-ethylmaleimide | - |
Homo sapiens | |
| 3.4.21.B28 | N-ethylmaleimide | - |
Mus musculus | |
| 3.4.21.B28 | Phenylmethylsulfonylfluoride | 1 mM | Homo sapiens | |
| 3.4.21.B28 | Phenylmethylsulfonylfluoride | 1 mM | Mus musculus | |
| 3.4.21.B28 | Talabostat | - |
Homo sapiens | |
| 3.4.21.B28 | Talabostat | - |
Mus musculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B28 | 0.029 | - |
L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | recombinant enzyme | Homo sapiens | |
| 3.4.21.B28 | 0.2 | - |
Gly-Pro-7-amido-4-trifluoromethylcoumarin | Km above 0.2 mM, wild type enzyme | Homo sapiens | |
| 3.4.21.B28 | 0.2 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | Km above 0.2 mM, wild type enzyme | Homo sapiens | |
| 3.4.21.B28 | 0.25 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | recombinant enzyme | Mus musculus | |
| 3.4.21.B28 | 0.323 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | recombinant enzyme | Homo sapiens | |
| 3.4.21.B28 | 1 | - |
Gly-Pro-7-amido-4-methylcoumarin | Km above 1.0 mM, wild type enzyme | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.B28 | membrane | - |
Gallus gallus | 16020 | - |
| 3.4.21.B28 | membrane | - |
Bos taurus | 16020 | - |
| 3.4.21.B28 | membrane | - |
Homo sapiens | 16020 | - |
| 3.4.21.B28 | membrane | - |
Mus musculus | 16020 | - |
| 3.4.21.B28 | membrane | - |
Xenopus laevis | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B28 | 95000 | - |
recombinant enzyme | Homo sapiens |
| 3.4.21.B28 | 96000 | - |
- |
Bos taurus |
| 3.4.21.B28 | 97000 | - |
2 * 97000 | Gallus gallus |
| 3.4.21.B28 | 97000 | - |
2 * 97000, wild type enzyme | Homo sapiens |
| 3.4.21.B28 | 160000 | - |
- |
Gallus gallus |
| 3.4.21.B28 | 170000 | - |
wild type enzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B28 | Gelatin + H2O | Gallus gallus | - |
? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | Bos taurus | - |
? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | Mus musculus | - |
? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | Xenopus laevis | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.B28 | Bos taurus | - |
- |
- |
| 3.4.21.B28 | Gallus gallus | - |
- |
- |
| 3.4.21.B28 | Homo sapiens | Q12884 | - |
- |
| 3.4.21.B28 | Mus musculus | P97321 | - |
- |
| 3.4.21.B28 | Xenopus laevis | Q91651 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.B28 | glycoprotein | - |
Gallus gallus |
| 3.4.21.B28 | glycoprotein | - |
Bos taurus |
| 3.4.21.B28 | glycoprotein | - |
Homo sapiens |
| 3.4.21.B28 | glycoprotein | - |
Mus musculus |
| 3.4.21.B28 | glycoprotein | - |
Xenopus laevis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.B28 | S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography | Homo sapiens |
| 3.4.21.B28 | S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography | Mus musculus |
| 3.4.21.B28 | S-200 gel filtration, hydroxylapatite column chromatography, Cibacron blue chromatography, and WGA-agarose column chromatography | Xenopus laevis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.B28 | breast cancer cell line | - |
Homo sapiens | - |
| 3.4.21.B28 | cervix carcinoma cell | - |
Homo sapiens | - |
| 3.4.21.B28 | embryo | - |
Gallus gallus | - |
| 3.4.21.B28 | fibroblast | - |
Homo sapiens | - |
| 3.4.21.B28 | LOX cell | - |
Homo sapiens | - |
| 3.4.21.B28 | MDA-MB-231 cell | - |
Homo sapiens | - |
| 3.4.21.B28 | melanoma cell line | - |
Homo sapiens | - |
| 3.4.21.B28 | melanoma cell line | - |
Mus musculus | - |
| 3.4.21.B28 | mesenchyme | transiently expressed in certain normal fetal mesenchymal tissues | Homo sapiens | - |
| 3.4.21.B28 | additional information | seprase is expressed during wound-healing, normal adult tissues as well as malignant epithelial, neural and haematopoietic cells are generally seprase-negative | Homo sapiens | - |
| 3.4.21.B28 | serum | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B28 | benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | - |
Gallus gallus | ? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | - |
Bos taurus | ? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | - |
Mus musculus | ? | - |
? | |
| 3.4.21.B28 | Gelatin + H2O | - |
Xenopus laevis | ? | - |
? | |
| 3.4.21.B28 | Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B28 | Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| 3.4.21.B28 | L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| 3.4.21.B28 | L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Mus musculus | L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| 3.4.21.B28 | L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg + H2O | FRET-peptide | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.B28 | homodimer | 2 * 97000 | Gallus gallus |
| 3.4.21.B28 | homodimer | 2 * 97000, wild type enzyme | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.B28 | FAPalpha | - |
Gallus gallus |
| 3.4.21.B28 | FAPalpha | - |
Bos taurus |
| 3.4.21.B28 | FAPalpha | - |
Homo sapiens |
| 3.4.21.B28 | FAPalpha | - |
Mus musculus |
| 3.4.21.B28 | FAPalpha | - |
Xenopus laevis |
| 3.4.21.B28 | fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Gallus gallus |
| 3.4.21.B28 | fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Bos taurus |
| 3.4.21.B28 | fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Homo sapiens |
| 3.4.21.B28 | fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Mus musculus |
| 3.4.21.B28 | fibroblast activation protein alpha | member of the group type II integral serine proteases, subfamily S9b, with gelatinase and N-terminal post-prolyl amino peptidase activities | Xenopus laevis |
| 3.4.21.B28 | seprase | - |
Gallus gallus |
| 3.4.21.B28 | seprase | - |
Bos taurus |
| 3.4.21.B28 | seprase | - |
Homo sapiens |
| 3.4.21.B28 | seprase | - |
Mus musculus |
| 3.4.21.B28 | seprase | - |
Xenopus laevis |
| 3.4.21.B28 | surface expressed protease | - |
Gallus gallus |
| 3.4.21.B28 | surface expressed protease | - |
Bos taurus |
| 3.4.21.B28 | surface expressed protease | - |
Homo sapiens |
| 3.4.21.B28 | surface expressed protease | - |
Mus musculus |
| 3.4.21.B28 | surface expressed protease | - |
Xenopus laevis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B28 | 37 | - |
- |
Gallus gallus |
| 3.4.21.B28 | 37 | - |
- |
Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B28 | 1.08 | - |
L-Ala-Pro-7-amido-4-trifluoromethylcoumarin | recombinant enzyme | Homo sapiens | |
| 3.4.21.B28 | 1.2 | - |
L-Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Gln-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | recombinant enzyme | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B28 | 7 | - |
wild type enzyme | Homo sapiens |
| 3.4.21.B28 | 7.5 | - |
- |
Bos taurus |
| 3.4.21.B28 | 7.6 | - |
- |
Gallus gallus |
| 3.4.21.B28 | 8.5 | - |
recombinant enzyme | Homo sapiens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.B28 | Homo sapiens | isoelectric focusing | - |
5 |
| 3.4.21.B28 | Bos taurus | isoelectric focusing | - |
5.68 |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.B28 | 0.00004 | - |
- |
Homo sapiens | Talabostat | |
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