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Literature summary extracted from

  • Kim, J.W.; Kim, Y.H.; Lee, H.S.; Yang, S.J.; Kim, Y.W.; Lee, M.H.; Kim, J.W.; Seo, N.S.; Park, C.S.; Park, K.H.
    Molecular cloning and biochemical characterization of the first archaeal maltogenic amylase from the hyperthermophilic archaeon Thermoplasma volcanium GSS1 (2007), Biochim. Biophys. Acta, 1774, 661-669.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expressed in Escherichia coli MC1061 Thermoplasma volcanium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.133 1.5
-
 maltoheptaose kinetic parameters for C14-labelled maltooligosaccharides Thermoplasma volcanium
3.2.1.133 1.6
-
 maltotetraose kinetic parameters for C14-labelled maltooligosaccharides Thermoplasma volcanium
3.2.1.133 3.6
-
 maltotriose kinetic parameters for C14-labelled maltooligosaccharides Thermoplasma volcanium
3.2.1.133 3.8
-
 maltohexaose kinetic parameters for C14-labelled maltooligosaccharides Thermoplasma volcanium
3.2.1.133 4.1
-
 soluble starch estimated for monomeric conformation, in presence of 1 M NaCl Thermoplasma volcanium
3.2.1.133 4.4
-
 maltopentaose kinetic parameters for C14-labelled maltooligosaccharides Thermoplasma volcanium
3.2.1.133 13
-
 soluble starch estimated for the high oligomer conformation Thermoplasma volcanium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.133 72060
-
 MALDI-TOF Thermoplasma volcanium
3.2.1.133 72090
-
 deduced from sequence Thermoplasma volcanium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.133 starch + H2O Thermoplasma volcanium transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O Thermoplasma volcanium GSS1 transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products alpha-maltose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Thermoplasma volcanium
-
 GSS1
-
3.2.1.133 Thermoplasma volcanium GSS1
-
 GSS1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 recombinant protein, gel filtration Thermoplasma volcanium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.133 additional information
-
 specific activity of starch hydrolysis estimated for the high oligomer conformation is about 7 U/mg and for monomeric state about 110 U/mg Thermoplasma volcanium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 acarbose + H2O
-
 Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 acarbose + H2O
-
 Thermoplasma volcanium GSS1 alpha-maltose + ?
-
 ?
3.2.1.133 beta-cyclodextrin + H2O high thermostability, substrate preference dependent on oligomeric state Thermoplasma volcanium alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 beta-cyclodextrin + H2O high thermostability, substrate preference dependent on oligomeric state Thermoplasma volcanium GSS1 alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 maltoheptaose + H2O
-
 Thermoplasma volcanium maltose + ?
-
 ?
3.2.1.133 maltohexaose + H2O
-
 Thermoplasma volcanium maltose + ?
-
 ?
3.2.1.133 maltopentaose + H2O
-
 Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 maltotetraose + H2O
-
 Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 maltotriose + H2O
-
 Thermoplasma volcanium alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 maltotriose + H2O
-
 Thermoplasma volcanium GSS1 alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 pullulan + H2O
-
 Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 soluble starch + H2O
-
 Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O
-
 Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products Thermoplasma volcanium alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O
-
 Thermoplasma volcanium GSS1 alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O transglycosylation pattern opposite to that of bacterial maltogenic amylases, predominant formation of alpha-1,4-glycosidic linked transfer products than of alpha-1,6-linked products Thermoplasma volcanium GSS1 alpha-maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 oligomer recombinant protein, high oligomer in solution, dissociation into dimer and monomer mixture by a high concentration of NaCl Thermoplasma volcanium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 glucan 1,4-alpha-maltohydrolase
-
 Thermoplasma volcanium
3.2.1.133 maltogenic amylase
-
 Thermoplasma volcanium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.133 75 80 high thermostability, dependent on oligomer structure of the enzyme, optimal activity at 75°C and 80°C for beta-cyclodextrin and soluble starch, half-lifes of 61 min at 75°C and of 24 min at 80°C Thermoplasma volcanium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.133 additional information
-
 high thermostability, half-lives of thermal inactivation and melting temperature tested Thermoplasma volcanium