Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bezsudnova, E.Y.; Sorokin, D.Y.; Tikhonova, T.V.; Popov, V.O.
    Thiocyanate hydrolase, the primary enzyme initiating thiocyanate degradation in the novel obligately chemolithoautotrophic halophilic sulfur-oxidizing bacterium Thiohalophilus thiocyanoxidans (2007), Biochim. Biophys. Acta, 1774, 1563-1570.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.8 acetate inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 azide inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 Borate inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 Br- inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 Cl- inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 Cyanate significant inhibition Thiohalophilus thiocyanatoxydans
3.5.5.8 nitrate inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 nitrite inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
3.5.5.8 sulfite inhibitory at 20 mM Thiohalophilus thiocyanatoxydans
4.2.1.104 chloride kinetics of cyanase activity at pH 7.5 and 37°C Thiohalophilus thiocyanatoxydans
4.2.1.104 Cyanate concentration above 4 mM Thiohalophilus thiocyanatoxydans
4.2.1.104 thiocyanate kinetics of cyanase activity at pH 7.5 and 37°C Thiohalophilus thiocyanatoxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.104 0.048
-
 Cyanate cyanate concentrations between 0.2 mM and 1 mM, in presence of 3 mM NaHCO3, at 37°C and pH 7.5 Thiohalophilus thiocyanatoxydans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.8 Co2+ contains 0.64 atoms Co2+ per heterotrimer Thiohalophilus thiocyanatoxydans
3.5.5.8 Fe2+ contains 0.41 atoms Fe2+ per heterotrimer Thiohalophilus thiocyanatoxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.8 17000
-
 2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration Thiohalophilus thiocyanatoxydans
3.5.5.8 19000
-
 2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration Thiohalophilus thiocyanatoxydans
3.5.5.8 29000
-
 2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration Thiohalophilus thiocyanatoxydans
3.5.5.8 130000
-
 gel filtration Thiohalophilus thiocyanatoxydans
3.5.5.8 140000
-
 SDS-PAGE Thiohalophilus thiocyanatoxydans
4.2.1.104 14000
-
 10 * 14000, estimated by gel filtration, SDS-PAGE, similar to apparent masses of cyanase of Escherichia coli Thiohalophilus thiocyanatoxydans
4.2.1.104 70000
-
 gel filtration Thiohalophilus thiocyanatoxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.104 NCO- + HCO3- + H+ Thiohalophilus thiocyanatoxydans cyanase activity in Thiohalophilus thiocyanoxidans, role in thiocyanate decomposition suggested NH3 + CO2 intermediates carbamate and CO2, spontaneous dissociation into NH3 and CO2 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.2.1.104 SDS stable after 2 h in 5% SDS at 37°C in 0.1 M potassium phosphate buffer Thiohalophilus thiocyanatoxydans

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.8 Thiohalophilus thiocyanatoxydans
-
-
-
4.2.1.104 Thiohalophilus thiocyanatoxydans
-
 halophilic sulfur-oxidizing gamma-proteobacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.8 DEAE-Sepharose column chromatography, Mono Q GI column chromatography, and Superdex 200 gel filtration Thiohalophilus thiocyanatoxydans
4.2.1.104 co-purified with thiocyanate hydrolase of Thiohalophilus thiocyanoxidans, gel filtration and SDS-PAGE Thiohalophilus thiocyanatoxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.8 0.03
-
 crude extract, in 0.1 M potassium phosphate buffer at 37°C and pH 7.5 Thiohalophilus thiocyanatoxydans
3.5.5.8 3.3
-
 after 110fold purification, in 0.1 M potassium phosphate buffer at 37°C and pH 7.5 Thiohalophilus thiocyanatoxydans
4.2.1.104 additional information
-
 activity assay in 0.1 M potassium phosphate buffer at pH 7.5 and 37°C with or without 3 mM NaHCO3, cyanate concentration between 0.2 mM and 2 mM, 0.2 to 1 microgram total protein per milliliter, measurement of NH3 formation Thiohalophilus thiocyanatoxydans
4.2.1.104 0.01
-
 in absence of bicarbonate Thiohalophilus thiocyanatoxydans
4.2.1.104 0.22
-
 in the presence of 3 mM bicarbonate and 2 mM cyanate Thiohalophilus thiocyanatoxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.8 additional information does not use thiosulfate as a substrate Thiohalophilus thiocyanatoxydans ?
-
 ?
3.5.5.8 thiocyanate + H2O
-
 Thiohalophilus thiocyanatoxydans carbonyl sulfide + NH3 + OH-
-
 ?
4.2.1.104 NCO- + HCO3- + H+ cyanase activity in Thiohalophilus thiocyanoxidans, role in thiocyanate decomposition suggested Thiohalophilus thiocyanatoxydans NH3 + CO2 intermediates carbamate and CO2, spontaneous dissociation into NH3 and CO2 ?
4.2.1.104 NCO- + HCO3- + H+ thiocyanate metabolism of Thiohalophilus thiocyanoxidans Thiohalophilus thiocyanatoxydans NH3 + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.8 heterohexamer 2 * 29000 + 2 * 19000 + 2 * 17000, gel filtration Thiohalophilus thiocyanatoxydans
4.2.1.104 decamer 10 * 14000, estimated by gel filtration, SDS-PAGE, similar to apparent masses of cyanase of Escherichia coli Thiohalophilus thiocyanatoxydans

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanase
-
 Thiohalophilus thiocyanatoxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.104 37
-
 assay at Thiohalophilus thiocyanatoxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.8 7.5 8
-
 Thiohalophilus thiocyanatoxydans
4.2.1.104 7.5
-
 assay at Thiohalophilus thiocyanatoxydans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.5.8 0.2
-
 Cyanate
-
 Thiohalophilus thiocyanatoxydans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.104 additional information
-
 kinetics of cyanase activity at pH 7.5 and 37°C, presence of 3 mM NaHCO3, cyanate concentrations between 0.2 and 2 mM Thiohalophilus thiocyanatoxydans additional information
4.2.1.104 6
-
-
 Thiohalophilus thiocyanatoxydans thiocyanate
4.2.1.104 15
-
-
 Thiohalophilus thiocyanatoxydans chloride