Literature summary extracted from

  • Xiang, H.; Masuo, S.; Hoshino, T.; Takaya, N.
    Novel family of cholesterol esterases produced by actinomycetes bacteria (2007), Biochim. Biophys. Acta, 1774, 112-120.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 Triton X-100 0.3%, 1.55fold activation Streptomyces sp.
3.1.1.13 cholic acid 0.3%, 1.5fold activation Streptomyces avermitilis
3.1.1.13 Tween 80 0.3%, 3fold activation Streptomyces avermitilis
3.1.1.13 Triton X-100 0.3%, 5fold activation Streptomyces griseus
3.1.1.13 Triton X-100 0.3%, 5fold activation Streptomyces avermitilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 2-mercaptoethanol 1 mM, 77% residual activity Streptomyces avermitilis
3.1.1.13 Ag+ 1 mM, 81% residual activity Streptomyces avermitilis
3.1.1.13 Ag+ 1 mM, 80% residual activity Streptomyces griseus
3.1.1.13 Ag+ 1 mM, 75% residual activity Streptomyces sp.
3.1.1.13 cholic acid 0.3%, 50% residual activity Streptomyces griseus
3.1.1.13 cholic acid 0.3%, 85% residual activity Streptomyces sp.
3.1.1.13 deoxycholic acid 0.3%, 40% residual activity Streptomyces avermitilis
3.1.1.13 deoxycholic acid 0.3%, 25% residual activity Streptomyces griseus
3.1.1.13 deoxycholic acid 0.3%, 33% residual activity Streptomyces sp.
3.1.1.13 dithiothreitol 1 mM, complete loss of activity Streptomyces avermitilis
3.1.1.13 dithiothreitol 1 mM, complete loss of activity Streptomyces griseus
3.1.1.13 dithiothreitol 1 mM, complete loss of activity Streptomyces sp.
3.1.1.13 Hg2+ 1 mM, complete loss of activity Streptomyces avermitilis
3.1.1.13 Hg2+ 1 mM, 40% residual activity Streptomyces griseus
3.1.1.13 Hg2+ 1 mM, 50% residual activity Streptomyces sp.
3.1.1.13 KCl 1 mM, 90% residual activity Streptomyces sp.
3.1.1.13 p-chloromercuribenzoic acid 1 mM, 75% residual activity Streptomyces avermitilis
3.1.1.13 p-chloromercuribenzoic acid 1 mM, 87% residual activity Streptomyces griseus
3.1.1.13 p-chloromercuribenzoic acid 1 mM, 78% residual activity Streptomyces sp.
3.1.1.13 Tween 20 0.3%, 25% residual activity Streptomyces avermitilis
3.1.1.13 Tween 20 0.3%, 50% residual activity Streptomyces griseus
3.1.1.13 Tween 20 0.3%, 45% residual activity Streptomyces sp.
3.1.1.13 Tween 80 0.3%, 90% residual activity Streptomyces griseus
3.1.1.13 Tween 80 0.3%, 30% residual activity Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.13 0.18
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 0.21
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 0.49
-
cholesteryl myristate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 1
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 0.8
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 0.35
-
cholesteryl myristate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 0.77
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 0.63
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 0.85
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 0.53
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 1.67
-
cholesteryl stearate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 2.86
-
cholesteryl palmitate pH 7.0, 37°C Streptomyces avermitilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.13 Cu2+ 1 mM, 120% of initial activity Streptomyces griseus
3.1.1.13 Cu2+ 1 mM, 120% of initial activity Streptomyces avermitilis
3.1.1.13 Cu2+ 1 mM, 120% of initial activity Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 120000
-
gel filtration Streptomyces avermitilis
3.1.1.13 153000
-
gel filtration Streptomyces griseus
3.1.1.13 19700
-
x * 19700, SDS-PAGE Streptomyces avermitilis
3.1.1.13 25700
-
x * 25700, SDS-PAGE Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Streptomyces griseus
-
-
-
3.1.1.13 Streptomyces sp. Q2AAT6
-
-
3.1.1.13 Streptomyces avermitilis Q82JC7 strain JCM5070
-
3.1.1.13 Streptomyces avermitilis JCM 5070 Q82JC7 strain JCM5070
-
3.1.1.13 Streptomyces sp. X9 Q2AAT6
-
-
3.1.1.13 Streptomyces griseus IFO 13350
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13
-
Streptomyces griseus
3.1.1.13
-
Streptomyces avermitilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 32.5
-
substrate cholesteryl linoleate, pH 7.0, 37°C Streptomyces griseus
3.1.1.13 33
-
substrate cholesteryl linoleate, pH 7.0, 37°C Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces griseus cholesterol + palmitate
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces avermitilis cholesterol + palmitate
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces sp. cholesterol + palmitate
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces avermitilis JCM 5070 cholesterol + palmitate
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces sp. X9 cholesterol + palmitate
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces griseus IFO 13350 cholesterol + palmitate
-
?
3.1.1.13 4-nitrophenyl palmitate + H2O
-
Streptomyces griseus 4-nitrophenol + palmitate
-
?
3.1.1.13 4-nitrophenyl palmitate + H2O
-
Streptomyces avermitilis 4-nitrophenol + palmitate
-
?
3.1.1.13 4-nitrophenyl palmitate + H2O
-
Streptomyces sp. 4-nitrophenol + palmitate
-
?
3.1.1.13 4-nitrophenyl palmitate + H2O
-
Streptomyces avermitilis JCM 5070 4-nitrophenol + palmitate
-
?
3.1.1.13 4-nitrophenyl palmitate + H2O
-
Streptomyces sp. X9 4-nitrophenol + palmitate
-
?
3.1.1.13 4-nitrophenyl palmitate + H2O
-
Streptomyces griseus IFO 13350 4-nitrophenol + palmitate
-
?
3.1.1.13 4-nitrophenyl laurate + H2O
-
Streptomyces griseus 4-nitrophenol + laurate
-
?
3.1.1.13 4-nitrophenyl laurate + H2O
-
Streptomyces avermitilis 4-nitrophenol + laurate
-
?
3.1.1.13 4-nitrophenyl laurate + H2O
-
Streptomyces sp. 4-nitrophenol + laurate
-
?
3.1.1.13 4-nitrophenyl laurate + H2O
-
Streptomyces avermitilis JCM 5070 4-nitrophenol + laurate
-
?
3.1.1.13 4-nitrophenyl laurate + H2O
-
Streptomyces sp. X9 4-nitrophenol + laurate
-
?
3.1.1.13 4-nitrophenyl laurate + H2O
-
Streptomyces griseus IFO 13350 4-nitrophenol + laurate
-
?
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces griseus cholesterol + acetate
-
?
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces avermitilis cholesterol + acetate
-
?
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces sp. cholesterol + acetate
-
?
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces avermitilis JCM 5070 cholesterol + acetate
-
?
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces sp. X9 cholesterol + acetate
-
?
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces griseus IFO 13350 cholesterol + acetate
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces griseus cholesterol + linoleate
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces avermitilis cholesterol + linoleate
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces sp. cholesterol + linoleate
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces avermitilis JCM 5070 cholesterol + linoleate
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces sp. X9 cholesterol + linoleate
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces griseus IFO 13350 cholesterol + linoleate
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Streptomyces griseus cholesterol + stearate
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Streptomyces avermitilis cholesterol + stearate
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Streptomyces sp. cholesterol + stearate
-
?
3.1.1.13 cholesteryl myristate + H2O
-
Streptomyces griseus cholesterol + myristate
-
?
3.1.1.13 cholesteryl myristate + H2O
-
Streptomyces avermitilis cholesterol + myristate
-
?
3.1.1.13 cholesteryl myristate + H2O
-
Streptomyces sp. cholesterol + myristate
-
?
3.1.1.13 4-nitrophenyl myristate + H2O
-
Streptomyces griseus 4-nitrophenol + myristate
-
?
3.1.1.13 4-nitrophenyl myristate + H2O
-
Streptomyces avermitilis 4-nitrophenol + myristate
-
?
3.1.1.13 4-nitrophenyl myristate + H2O
-
Streptomyces sp. 4-nitrophenol + myristate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 oligomer x * 19700, SDS-PAGE Streptomyces avermitilis
3.1.1.13 oligomer x * 25700, SDS-PAGE Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.13 1.73
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 23.3
-
cholesteryl stearate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 24.7
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 12.4
-
cholesteryl myristate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 13.3
-
cholesteryl myristate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 2.52
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 25.6
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 2.56
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 3.43
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces griseus
3.1.1.13 15.7
-
cholesteryl palmitate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 1.44
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces avermitilis
3.1.1.13 3.86
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces griseus