EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | additional information | weak stimulation by addition of recombinant human PCNA | Podospora anserina |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.30.2 | His-tag fusion protein expressed in Escherichia coli | Podospora anserina |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | Ca2+ | weak inhibition at 10 mM, 50% inhibition at 50 mM | Podospora anserina | |
3.1.30.2 | EDTA | inactive in the presence of EDTA | Podospora anserina | |
3.1.30.2 | KCl | weak inhibition up to 200 mM | Podospora anserina | |
3.1.30.2 | NaCl | 10fold inhibition at 25 mM | Podospora anserina |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.30.2 | mitochondrion | - |
Podospora anserina | 5739 | - |
3.1.30.2 | soluble | N-terminal His-tagged protein mainly found in the culture supernatant | Podospora anserina | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | Mg2+ | optimum concentration 10 mM, inhibitory at higher concentrations | Podospora anserina |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 49000 | - |
gel filtration, SDS-PAGE | Podospora anserina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | dsDNA + H2O | Podospora anserina | - |
? | - |
? | |
3.1.30.2 | flap DNA + H2O | Podospora anserina | - |
? | - |
? | |
3.1.30.2 | additional information | Podospora anserina | recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand | ? | - |
? | |
3.1.30.2 | ssDNA + H2O | Podospora anserina | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.30.2 | Podospora anserina | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.30.2 | recombinant protein using His-tag | Podospora anserina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | dsDNA + H2O | - |
Podospora anserina | ? | - |
? | |
3.1.30.2 | flap DNA + H2O | - |
Podospora anserina | ? | - |
? | |
3.1.30.2 | M13 flap DNA + H2O | - |
Podospora anserina | ? | small fragment of 5-10 nucleotides | ? | |
3.1.30.2 | M13 mp19 (+) DNA + H2O | - |
Podospora anserina | ? | small fragment of 5-8 nucleotides | ? | |
3.1.30.2 | additional information | recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand | Podospora anserina | ? | - |
? | |
3.1.30.2 | additional information | linear flap structures with tails of more than 20 nucleotides and shorter duplex regions are not hydrolyzed | Podospora anserina | ? | - |
? | |
3.1.30.2 | pUC19 DNA + H2O | relaxation of the supercoiled DNA and cutting of the open circular DNA to a linear form | Podospora anserina | ? | - |
? | |
3.1.30.2 | ssDNA + H2O | - |
Podospora anserina | ? | - |
? | |
3.1.30.2 | ssDNA + H2O | - |
Podospora anserina | ? | small fragment of 8-20 nucleotides | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.30.2 | monomer | 1 * 49000, SDS-PAGE, native mass by gel filtration | Podospora anserina |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.30.2 | NUC49 | - |
Podospora anserina |
3.1.30.2 | rNUC49 | recombinant NUC49 from Escherichia coli | Podospora anserina |