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Literature summary extracted from

  • Laquel-Robert, P.; Sellem, C.H.; Sainsard-Chanet, A.; Castroviejo, M.
    Identification and biochemical analysis of a mitochondrial endonuclease of Podospora anserina related to curved-DNA binding proteins (2007), Biochim. Biophys. Acta, 1770, 527-542.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.30.2 additional information weak stimulation by addition of recombinant human PCNA Podospora anserina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.30.2 His-tag fusion protein expressed in Escherichia coli Podospora anserina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 Ca2+ weak inhibition at 10 mM, 50% inhibition at 50 mM Podospora anserina
3.1.30.2 EDTA inactive in the presence of EDTA Podospora anserina
3.1.30.2 KCl weak inhibition up to 200 mM Podospora anserina
3.1.30.2 NaCl 10fold inhibition at 25 mM Podospora anserina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.30.2 mitochondrion
-
Podospora anserina 5739
-
3.1.30.2 soluble N-terminal His-tagged protein mainly found in the culture supernatant Podospora anserina
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 Mg2+ optimum concentration 10 mM, inhibitory at higher concentrations Podospora anserina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.2 49000
-
gel filtration, SDS-PAGE Podospora anserina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.2 dsDNA + H2O Podospora anserina
-
?
-
?
3.1.30.2 flap DNA + H2O Podospora anserina
-
?
-
?
3.1.30.2 additional information Podospora anserina recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand ?
-
?
3.1.30.2 ssDNA + H2O Podospora anserina
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Podospora anserina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.2 recombinant protein using His-tag Podospora anserina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.2 dsDNA + H2O
-
Podospora anserina ?
-
?
3.1.30.2 flap DNA + H2O
-
Podospora anserina ?
-
?
3.1.30.2 M13 flap DNA + H2O
-
Podospora anserina ? small fragment of 5-10 nucleotides ?
3.1.30.2 M13 mp19 (+) DNA + H2O
-
Podospora anserina ? small fragment of 5-8 nucleotides ?
3.1.30.2 additional information recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand Podospora anserina ?
-
?
3.1.30.2 additional information linear flap structures with tails of more than 20 nucleotides and shorter duplex regions are not hydrolyzed Podospora anserina ?
-
?
3.1.30.2 pUC19 DNA + H2O relaxation of the supercoiled DNA and cutting of the open circular DNA to a linear form Podospora anserina ?
-
?
3.1.30.2 ssDNA + H2O
-
Podospora anserina ?
-
?
3.1.30.2 ssDNA + H2O
-
Podospora anserina ? small fragment of 8-20 nucleotides ?

Subunits

EC Number Subunits Comment Organism
3.1.30.2 monomer 1 * 49000, SDS-PAGE, native mass by gel filtration Podospora anserina

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 NUC49
-
Podospora anserina
3.1.30.2 rNUC49 recombinant NUC49 from Escherichia coli Podospora anserina