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Literature summary extracted from

  • Saehuan, C.; Rojanarata, T.; Wiyakrutta, S.; McLeish, M.J.; Meevootisom, V.
    Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201 (2007), Biochim. Biophys. Acta, 1770, 1585-1592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 expressed in Escherichia coli BL21(DE3) cells Pseudomonas stutzeri
4.1.1.7 expressed in Escherichia coli BL21(DE3) cells Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.0069
-
 benzaldehyde in the presence of NADP+, at 25°C and pH 8.5 Pseudomonas stutzeri
1.2.1.16 0.0071
-
 benzaldehyde in the presence of NAD+, at 25°C and pH 8.5 Pseudomonas stutzeri
1.2.1.16 0.997
-
 NAD+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri
1.2.1.16 6.14
-
 NADP+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri
4.1.1.7 0.44
-
 benzoylformate recombinant, his-tagged enzyme at pH 6.0 and 35°C Pseudomonas stutzeri
4.1.1.7 0.69
-
 benzoylformate wild type enzyme at pH 6.0 and 35°C Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.7 55000
-
 4 * 55000, gel filtration Pseudomonas stutzeri
4.1.1.7 220000
-
 gel filtration Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 benzaldehyde + NAD+ + H2O Pseudomonas stutzeri
-
 benzoate + NADH + H+
-
 ?
1.2.1.16 benzaldehyde + NAD+ + H2O Pseudomonas stutzeri ST-201
-
 benzoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Pseudomonas stutzeri
-
-
-
1.2.1.16 Pseudomonas stutzeri ST-201
-
-
-
4.1.1.7 Pseudomonas stutzeri
-
-
-
4.1.1.7 Pseudomonas stutzeri ST-201
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography Pseudomonas stutzeri
4.1.1.7 Phenyl Sepharose FF column chromatography and Q Sepharose FF column chromatography Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 benzaldehyde + NAD+ + H2O
-
 Pseudomonas stutzeri benzoate + NADH + H+
-
 ?
1.2.1.16 benzaldehyde + NAD+ + H2O
-
 Pseudomonas stutzeri ST-201 benzoate + NADH + H+
-
 ?
1.2.1.16 benzaldehyde + NADP+ + H2O weaker reaction compared to NAD+ Pseudomonas stutzeri benzoate + NADPH + H+
-
 ?
1.2.1.16 benzaldehyde + NADP+ + H2O weaker reaction compared to NAD+ Pseudomonas stutzeri ST-201 benzoate + NADPH + H+
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas stutzeri benzaldehyde + CO2
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas stutzeri ST-201 benzaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.7 homotetramer 4 * 55000, gel filtration Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 NAD+/NADP+-dependent benzaldehyde dehydrogenase
-
 Pseudomonas stutzeri
4.1.1.7 BFDC
-
 Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.7 35 50
-
 Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.16 6.4
-
 benzaldehyde in the presence of NADP+, at 25°C and pH 8.5 Pseudomonas stutzeri
1.2.1.16 40
-
 NADP+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri
1.2.1.16 57
-
 benzaldehyde in the presence of NAD+, at 25°C and pH 8.5 Pseudomonas stutzeri
1.2.1.16 176
-
 NAD+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri
4.1.1.7 222
-
 benzoylformate wild type enzyme at pH 6.0 and 35°C Pseudomonas stutzeri
4.1.1.7 284
-
 benzoylformate recombinant, his-tagged enzyme at pH 6.0 and 35°C Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.7 6
-
-
 Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+ preferred cofactor Pseudomonas stutzeri
1.2.1.16 NADP+ NAD+ is preferred over NADP+ Pseudomonas stutzeri
4.1.1.7 thiamine diphosphate dependent Pseudomonas stutzeri