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Literature summary extracted from

  • Kestwal, R.M.; Bhide, S.V.
    Purification of beta-galactosidase from Erythrina indica: involvement of tryptophan in active site (2007), Biochim. Biophys. Acta, 1770, 1506-1512.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 beta-D-galactose product inhibition Erythrina variegata
3.2.1.23 diethyldicarbonate
-
 Erythrina variegata
3.2.1.23 Hg2+ complete inhibition at 5 mM Erythrina variegata
3.2.1.23 additional information no inhibition by PMSF, DTNB, phenyl glyoxal, N-acetyl imidazole, and N,N'-dicyclohexyl carbodiimide Erythrina variegata
3.2.1.23 N-bromosuccimide strong inhibition, attacks a tryptophan residue Erythrina variegata
3.2.1.23 Zn2+ complete inhibition at 5 mM Erythrina variegata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 2.6
-
 4-nitrophenyl-beta-D-galactopyranoside pH 4.6, 30°C Erythrina variegata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 74000
-
 gel filtration Erythrina variegata
3.2.1.23 78000
-
 SDS-PAGE Erythrina variegata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Erythrina variegata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein the enzyme is less glycosylated than other glycosidases Erythrina variegata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 native enzyme 135fold by ammonium sulfate fractionation, concanavalin A affinity chromatography, anion and cation exchange chromatography, and gel filtration to homogeneity Erythrina variegata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 seed
-
 Erythrina variegata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 109
-
 purified enzyme Erythrina variegata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Erythrina variegata 4-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer
-
 Erythrina variegata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Erythrina variegata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.4
-
-
 Erythrina variegata