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Literature summary extracted from

  • Hellstroem, J.L.; Vehniaeinen, M.; Mustonen, M.; Loevgren, T.; Lamminmaeki, U.; Hellman, J.
    Unfolding of the immunoglobulin light and heavy chains is required for the enzymatic removal of N-terminal pyroglutamyl residues (2006), Biochim. Biophys. Acta, 1764, 1735-1740.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.19.3 dithiothreitol
-
 Pyrococcus furiosus
3.4.19.3 methanol a methanol based deblocking solution preserves enzymatic activity, provides conditions compatible with sequencing and enhances deblocking of electroblotted samples Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.3 PGAP expressed from a pET3a-series vector in Escherichia coli BL21(DE) cells Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3 by cation exchange chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 L-pyroglutamyl p-nitroanilide + H2O
-
 Pyrococcus furiosus L-pyroglutamate + p-nitroaniline
-
 ?
3.4.19.3 additional information PGAP performs enzymatic deblocking of pyroglutamylated immunoglobulins. Unfolding of immunoglobulins is required for excision of the pyroglutamate. The enzyme requires the substrate in an extended conformation, which is not fullfiled in the native form of immunoglobulins Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.19.3 PGAP
-
 Pyrococcus furiosus
3.4.19.3 pyroglutamate aminopeptidase
-
 Pyrococcus furiosus