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Literature summary extracted from

  • Porcelli, M.; Cacciapuoti, G.; Fusco, S.; Iacomino, G.; Gambacorta, A.; De Rosa, M.; Zappia, V.
    S-Adenosylhomocysteine hydrolase from the thermophilic archaeon Sulfolobus solfataricus: purification, physico-chemical and immunological properties. (1993), Biochim. Biophys. Acta, 1164, 179-188.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 15
-
 S-adenosyl-L-homocysteine
-
 Saccharolobus solfataricus
3.13.2.1 150
-
 L-homocysteine
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 47000
-
 4 * 47000, SDS-PAGE Saccharolobus solfataricus
3.13.2.1 190000
-
 gel filtration Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.13.2.1 Ethanol 50%, 48 h at room temperature, no loss of activity Saccharolobus solfataricus
3.13.2.1 guanidine-HCl 6 M, 48 h at room temperature, no loss of activity Saccharolobus solfataricus
3.13.2.1 Methanol 50%, 48 h at room temperature, no loss of activity Saccharolobus solfataricus
3.13.2.1 SDS 0.075%, 48 h at room temperature, no loss of activity Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 0.161
-
-
 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -20°C, 20 mM Tris-HC1, pH 8, containing 1 mM EDTA, stable for at least 2 months Saccharolobus solfataricus
3.13.2.1 4°C, the enzyme is spontaneously inactivated in one month, in the presence of 10% glycerol the stability of the enzyme increases up to 80% Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 L-homocysteine + adenosine
-
 Saccharolobus solfataricus S-adenosyl-L-homocysteine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 tetramer 4 * 47000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.2.1 75
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.13.2.1 55 90 about 50% of maximal activity 55°C and at 90°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.2.1 25
-
 60 min, 20% loss of activity Saccharolobus solfataricus
3.13.2.1 50
-
 16 h, stable Saccharolobus solfataricus
3.13.2.1 70
-
 16 h, 40% loss of activity Saccharolobus solfataricus
3.13.2.1 75
-
 60 min, almost complete loss of activity Saccharolobus solfataricus
3.13.2.1 90
-
 2 h, 80% loss of activity Saccharolobus solfataricus
3.13.2.1 95
-
 melting temperature Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.2.1 7.5
-
-
 Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.2.1 NAD+ the enzyme contains four molecules of tightly-bound NAD+ per tetramer of which about 40% is in the reduced form Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.13.2.1 Saccharolobus solfataricus
-
-
 5.3