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Literature summary extracted from

  • Manukhov, I.V.; Mamaeva, D.V.; Morozova, E.A.; Rastorguev, S.M.; Faleev, N.G.; Demidkina, T.V.; Zavilgelsky, G.B.
    L-methionine gamma-lyase from Citrobacter freundii: cloning of the gene and kinetic parameters of the enzyme (2006), Biochemistry (Moscow), 71, 361-369.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.11 synthesis presence of a hybrid plasmid in Escherichia coli K12 containing the enzyme gene leads to a decrease in efficiciency of EcoKI restriction Citrobacter freundii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Escherichia coli Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 0.16
-
 L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 0.17
-
 S-ethyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 0.18
-
 S-Benzyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 0.5
-
 S-ethyl-L-homocysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 0.7
-
 L-methionine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 0.71
-
 S-methyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 1
-
 DL-homocysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 6.7
-
 L-Gly(vinyl) 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 56.5
-
 DL-homoserine 30°C, pH 8.0 Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Citrobacter freundii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11
-
 Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 DL-homocysteine + H2O
-
 Citrobacter freundii hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 DL-homoserine + H2O
-
 Citrobacter freundii NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-cysteine
-
 Citrobacter freundii 2-oxopropanoate + NH3 + H2S
-
 ?
4.4.1.11 L-Gly(vinyl) + H2O
-
 Citrobacter freundii ?
-
 ?
4.4.1.11 L-methionine
-
 Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O
-
 Citrobacter freundii thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-ethyl-L-homocysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 S-methyl-L-cysteine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.11 0.5
-
 DL-homoserine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 1.9
-
 L-Gly(vinyl) 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 2.3
-
 L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 4.6
-
 S-methyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 5
-
 S-ethyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 6.2
-
 L-methionine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 6.8
-
 S-ethyl-L-homocysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 8.2
-
 S-Benzyl-L-cysteine 30°C, pH 8.0 Citrobacter freundii
4.4.1.11 8.5
-
 DL-homocysteine 30°C, pH 8.0 Citrobacter freundii