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Literature summary extracted from

  • Gombos, L.; Kardos, J.; Patthy, A.; Medveczky, P.; Szilagyi, L.; Malnasi-Csizmadia, A.; Graf, L.
    Probing conformational plasticity of the activation domain of trypsin: the role of glycine hinges (2008), Biochemistry, 47, 1675-1684.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.4 expressed in Escherichia coli strain BL21(DE3)pLysS Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.4 R193A slightly reduced activity Homo sapiens
3.4.21.4 R193G completely resistant to chymotryptic degradation Homo sapiens
3.4.21.4 R193G/G142A reduced activity Homo sapiens
3.4.21.4 R193G/G184A reduced activity Homo sapiens
3.4.21.4 R193G/G19A reduced activity, completely resistant to chymotryptic degradation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 additional information trypsin 4 is completely resistant to chymotryptic degradation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.0141
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 0.0203
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 0.0266
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 0.0298
-
 Z-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 0.0788
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 0.346
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 Q Sepharose Fast Flow column chromatography and Phenyl Sepharose Fast Flow column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 4-methylumbelliferyl-4-guanidinobenzoate + H2O
-
 Homo sapiens 4-methylumbelliferol + 4-guanidinobenzoate
-
 ?
3.4.21.4 Z-Gly-Pro-Arg-p-nitroanilide + H2O
-
 Homo sapiens Z-Gly-Pro-Arg + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 trypsin 4
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 3.86
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 20 50 Z-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 29.2
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 51.5
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 54.1
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 83.6
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
3.4.21.4 88.2
-
 Z-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens