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Literature summary extracted from

  • Narayanan, B.C.; Niu, W.; Han, Y.; Zou, J.; Mariano, P.S.; Dunaway-Mariano, D.; Herzberg, O.
    Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily (2008), Biochemistry, 47, 167-182.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.112 expressed in Escherichia coli BL21 (DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.112 hanging drop vapour diffusion method, aliquots of 10 mg/ml protein in 5 mM MgCl2, 5 mM phosphonopyruvate and 10 mM NaHEPES (pH 7.0) mixed with equal volumes of reservoir solution containing 12% polyethylene glycol 20000 and 0.1 M MES (pH 6.0) Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.112 H235A the kcat value for catalysis of oxaloacetate decarboxylation is less than an order of magnitude smaller compared to the wild type enzyme Pseudomonas aeruginosa
4.1.1.112 H235Q the kcat value for catalysis of oxaloacetate decarboxylation is less than an order of magnitude smaller compared to the wild type enzyme Pseudomonas aeruginosa
4.1.1.112 Y212F 25fold reduction of the Km value compared to the wild type enzyme Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.112 3,3-difluoroxaloacetate
-
 Pseudomonas aeruginosa
4.1.1.112 Acetopyruvate
-
 Pseudomonas aeruginosa
4.1.1.112 alpha-ketovalerate
-
 Pseudomonas aeruginosa
4.1.1.112 oxalate
-
 Pseudomonas aeruginosa
4.1.1.112 Phosphonopyruvate
-
 Pseudomonas aeruginosa
4.1.1.112 pyruvate
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.112 0.087
-
 oxaloacetate mutant enzyme Y212F, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa
4.1.1.112 0.63
-
 3-methyloxaloacetate wild type enzyme, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa
4.1.1.112 1.02
-
 oxaloacetate mutant enzyme H235A, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa
4.1.1.112 2.2
-
 oxaloacetate wild type enzyme, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa
4.1.1.112 2.5
-
 oxaloacetate mutant enzyme H235Q, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.112 Mg2+ cofactor Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Pseudomonas aeruginosa Q9HUU1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112 DEAE-cellulose column chromatography, phenyl-Sepharose columnn chromatography, and butyl-Sepharose column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 3-methyloxaloacetate
-
 Pseudomonas aeruginosa 2-oxobutanoate + CO2
-
 ?
4.1.1.112 Oxaloacetate
-
 Pseudomonas aeruginosa Pyruvate + CO2
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.1.112 dimer dimer of dimers, X-ray crystallography Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
4.1.1.112 PA4872 member of the PEP mutase/isocitrate lyase superfamily Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.112 250
-
 3-methyloxaloacetate in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa
4.1.1.112 7500
-
 oxaloacetate in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.112 0.043
-
 oxalate
-
 Pseudomonas aeruginosa
4.1.1.112 0.45
-
 3,3-difluoroxaloacetate
-
 Pseudomonas aeruginosa
4.1.1.112 1.09
-
 Acetopyruvate
-
 Pseudomonas aeruginosa
4.1.1.112 3
-
 Phosphonopyruvate
-
 Pseudomonas aeruginosa
4.1.1.112 6.7
-
 alpha-ketovalerate
-
 Pseudomonas aeruginosa
4.1.1.112 7.2
-
 pyruvate
-
 Pseudomonas aeruginosa