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Literature summary extracted from

  • Ghanem, E.; Li, Y.; Xu, C.; Raushel, F.M.
    Characterization of a phosphodiesterase capable of hydrolyzing EA 2192, the most toxic degradation product of the nerve agent VX (2007), Biochemistry, 46, 9032-9040.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.4.2 biotechnology efficient selection system for the identification of phosphotriesterase mutants with enhanced catalytic properties for selected organophosphate substrates Klebsiella aerogenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 expression in Escherichia coli Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.2 0.06
-
 dimethyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 0.4
-
 4-nitrophenyl ethylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 0.5
-
 diethyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 0.74
-
 ethyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 0.9
-
 bis(4-nitrophenyl) phosphate
-
 Klebsiella aerogenes
3.1.4.2 0.9
-
 ethyl 4-nitrophenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 1.3
-
 4-nitrophenyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 1.8
-
 4-nitrophenyl phenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 2.6
-
 methyl 4-nitrophenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 3.3
-
 isobutyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 6.5
-
 isopropyl 4-nitrophenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 13
-
 4-nitrophenyl phenyl phosphonate
-
 Klebsiella aerogenes
3.1.4.2 19
-
 isopropyl methylphosphonate
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Co2+
-
 Klebsiella aerogenes
3.1.4.2 Mn2+ specific acitivity towards bis(4-nitrophenyl) phosphate is enhanced 4fold in presence of MnCl2 Klebsiella aerogenes
3.1.4.2 Zn2+ specific acitivity towards bis(4-nitrophenyl) phosphate is enhanced 2fold in presence of ZnCl2 Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.2 30840
-
 calculated for one subunit Klebsiella aerogenes
3.1.4.2 31000
-
 determined by SDS-PAGE Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.2 size exclusion column, anion-exchange column Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 1,2,2-trimethylpropyl methylphosphonate + H2O
-
 Klebsiella aerogenes 3,3-dimethylbutan-2-ol + methylphosphonate
-
 ir
3.1.4.2 4-nitrophenyl ethylphosphonate + H2O
-
 Klebsiella aerogenes 4-nitrophenyl + ethylphosphonate
-
 ir
3.1.4.2 4-nitrophenyl methylphosphonate + H2O
-
 Klebsiella aerogenes 4-nitrophenyl + methylphosphonate
-
 ir
3.1.4.2 4-nitrophenyl phenyl phosphate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + phenyl phosphate
-
 ir
3.1.4.2 4-nitrophenyl phenyl phosphonate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + phenyl phosphonate
-
 ir
3.1.4.2 4-nitrophenylphosphate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + phosphate
-
 ir
3.1.4.2 bis(4-nitrophenyl) phosphate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + phosphate
-
 ir
3.1.4.2 diethyl phosphate + H2O
-
 Klebsiella aerogenes ethanol + ethyl phosphate
-
 ir
3.1.4.2 dimethyl 4-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes ?
-
 ir
3.1.4.2 dimethyl phosphate + H2O
-
 Klebsiella aerogenes methanol + methyl phosphate
-
 ir
3.1.4.2 ethyl 4-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + ethyl phosphate
-
 ir
3.1.4.2 ethyl demeton S
-
 Klebsiella aerogenes ?
-
 ?
3.1.4.2 ethyl methyl 4-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes ?
-
 ir
3.1.4.2 ethyl methylphosphonate + H2O
-
 Klebsiella aerogenes ethanol + methylphosphonate
-
 ir
3.1.4.2 isobutyl methylphosphonate + H2O
-
 Klebsiella aerogenes isobutanol + methylphosphonate
-
 ir
3.1.4.2 isopropyl 4-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + isopropyl phosphate
-
 ir
3.1.4.2 isopropyl methylphosphonate + H2O
-
 Klebsiella aerogenes isopropanol + methylphosphonate
-
 ir
3.1.4.2 methyl 4-nitrophenyl phosphate + H2O
-
 Klebsiella aerogenes 4-nitrophenol + methyl phosphate
-
 ir
3.1.4.2 O-ethyl S-[2-(ethylsulfanyl)ethyl] hydrogen thiophosphate + H2O
-
 Klebsiella aerogenes ?
-
 ir
3.1.4.2 paraoxon + H2O
-
 Klebsiella aerogenes 4-nitrophenol + diethyl phosphate
-
 ir
3.1.4.2 phenyl methylphosphonate + H2O
-
 Klebsiella aerogenes phenol + methylphosphonate
-
 ir
3.1.4.2 S-[2-(ethylsulfanyl)ethyl] O,O-dimethyl thiophosphate + H2O
-
 Klebsiella aerogenes ?
-
 ir
3.1.4.2 S-[2-(ethylsulfanyl)ethyl] O-methyl hydrogen thiophosphate + H2O
-
 Klebsiella aerogenes ?
-
 ir
3.1.4.2 S-[2-[bis(1-methylethyl)amino]ethyl] O-ethyl hydrogen thiophosphate + H2O
-
 Klebsiella aerogenes ?
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 glycerophosphodiesterase
-
 Klebsiella aerogenes
3.1.4.2 GpdQ
-
 Klebsiella aerogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.2 0.001
-
 isopropyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 0.001
-
 isobutyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 0.0012
-
 ethyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 0.0012
-
 O-ethyl S-[2-(ethylsulfanyl)ethyl] hydrogen thiophosphate
-
 Klebsiella aerogenes
3.1.4.2 0.0031
-
 phenyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 0.005
-
 dimethyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 0.0074
-
 diethyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 0.5
-
 4-nitrophenyl ethylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 1
-
 isopropyl 4-nitrophenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 1.1
-
 S-[2-[bis(1-methylethyl)amino]ethyl] O-ethyl hydrogen thiophosphate
-
 Klebsiella aerogenes
3.1.4.2 1.6
-
 4-nitrophenyl methylphosphonate
-
 Klebsiella aerogenes
3.1.4.2 2
-
 ethyl 4-nitrophenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 2.8
-
 4-nitrophenyl phenyl phosphonate
-
 Klebsiella aerogenes
3.1.4.2 4
-
 4-nitrophenyl phenyl phosphate
-
 Klebsiella aerogenes
3.1.4.2 6
-
 bis(4-nitrophenyl) phosphate
-
 Klebsiella aerogenes
3.1.4.2 7.3
-
 methyl 4-nitrophenyl phosphate
-
 Klebsiella aerogenes