EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.2.12 | structure of wild-type enzyme, wild type enzyme in complex with ryruvated D-mannose and mutant enzyme R612A, hanging-drop vapor diffusion | Bacillus sp. GL1 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.2.12 | R313A | KM-value for xanthan is 1.5fold higher than wild type value. kcat for xanthan is 219fold lower than wild-type value | Bacillus sp. GL1 |
4.2.2.12 | R612A | KM-value for xanthan is 11.6fold higher than wild type value. kcat for xanthan is 2.8fold higher than wild-type value | Bacillus sp. GL1 |
4.2.2.12 | Y315F | KM-value for xanthan is 1.8fold higher than wild type value. kcat for xanthan is 1.3fold lower than wild-type value | Bacillus sp. GL1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.12 | 4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose | - |
Bacillus sp. GL1 | |
4.2.2.12 | D-mannose | - |
Bacillus sp. GL1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.12 | Bacillus sp. GL1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.12 | expression of wild-type and mutant enzymes in Escherichia coli | Bacillus sp. GL1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.12 | xanthan | - |
Bacillus sp. GL1 | D-xylooligosaccharides | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.12 | 9.92 | - |
Xanthan | 30°C, pH 5.5, mutant enzyme R313A | Bacillus sp. GL1 | |
4.2.2.12 | 1730 | - |
Xanthan | 30°C, pH 5.5, Y315F | Bacillus sp. GL1 | |
4.2.2.12 | 2170 | - |
Xanthan | 30°C, pH 5.5, wild-type enzyme | Bacillus sp. GL1 | |
4.2.2.12 | 6120 | - |
Xanthan | 30°C, pH 5.5, R612A | Bacillus sp. GL1 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.12 | 2.21 | - |
4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose | 30°C, pH 5.5, wild type enzyme | Bacillus sp. GL1 | |
4.2.2.12 | 4 | 10 | D-mannose | 30°C, pH 5.5,mutant enzyme R612A | Bacillus sp. GL1 | |
4.2.2.12 | 19.2 | - |
4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose | 30°C, pH 5.5,mutant enzyme R612A | Bacillus sp. GL1 | |
4.2.2.12 | 837 | - |
D-mannose | 30°C, pH 5.5, wild type enzyme | Bacillus sp. GL1 |