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Literature summary extracted from

  • Frankel, B.A.; Tong, Y.; Bentley, M.L.; Fitzgerald, M.C.; McCafferty, D.G.
    Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA (2007), Biochemistry, 46, 7269-7278.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 wild-type and variant SrtA proteins Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.70 C184S 2700fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 D185A 1.3 fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 D186A 1.8fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 H120A 96000fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 H120Q 36000fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 I182A mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 28fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 I182S 74fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 L181A mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 7.6fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 R197A 540fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 R197K 1000fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 T180A mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 14fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus
3.4.22.70 T183A 1200fold decrease in kcat/Km compared to wild-type value Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.70 0.79
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 3.8
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 4.7
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 6.7
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 8.5
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 10.6
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide wild-type enzyme Staphylococcus aureus
3.4.22.70 11.2
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 Abz-LPETG-Dap(Dnp)-NH2 + GGGGG
-
 Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.70 0.000019
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 0.0003
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 0.00046
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 0.0006
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 0.69
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide wild-type enzyme Staphylococcus aureus
3.4.22.70 0.72
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus
3.4.22.70 0.78
-
 N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide mutant enzyme Staphylococcus aureus