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Literature summary extracted from

  • Omi, R.; Goto, M.; Miyahara, I.; Manzoku, M.; Ebihara, A.; Hirotsu, K.
    Crystal structure of monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8 (2007), Biochemistry, 46, 12618-12627.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.15 expressed in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.15 vapour diffusion method with 0.68 M sodium malonate and 100 mM Tris-HCl (pH 8.5) Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.15 0.27
-
 L-histidinol phosphate
-
 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.15 Fe2+ part of the trinuclear metal center Thermus thermophilus
3.1.3.15 Zn2+ part of the trinuclear metal center Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.15 29500
-
 4 * 29500, dynamic light scattering Thermus thermophilus
3.1.3.15 29976
-
 4 * 29976, calculated from sequence of cDNA Thermus thermophilus
3.1.3.15 118000
-
 dynamic light scattering Thermus thermophilus
3.1.3.15 119900
-
 calculated from sequence of cDNA Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.15 Thermus thermophilus
-
-
-
3.1.3.15 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.15
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.15 L-histidinol phosphate + H2O
-
 Thermus thermophilus L-histidinol + phosphate
-
 ?
3.1.3.15 L-histidinol phosphate + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 L-histidinol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.15 homotetramer 4 * 29500, dynamic light scattering Thermus thermophilus
3.1.3.15 homotetramer 4 * 29976, calculated from sequence of cDNA Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.15 HisPPase
-
 Thermus thermophilus
3.1.3.15 histidinol phosphate phosphatase
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.15 0.93
-
 L-histidinol phosphate
-
 Thermus thermophilus