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Literature summary extracted from

  • Karkehabadi, S.; Satagopan, S.; Taylor, T.C.; Spreitzer, R.J.; Andersson, I.
    Structural analysis of altered large-subunit loop-6/carboxy-terminus interactions that influence catalytic efficiency and CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase (2007), Biochemistry, 46, 11080-11089.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.39 hanging drop vapour diffusion method using 50 mM HEPES (pH 7.5), 0.05-0.2 M NaCl, 7-12% poly(ethylene glycol) 4000, 10 mM NaHCO3, and 5 mM MgCl2 Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 D473E altered CO2/O2 specificity Chlamydomonas reinhardtii
4.1.1.39 G344S decrease in CO2/O2 specificity Chlamydomonas reinhardtii
4.1.1.39 T342I altered CO2/O2 specificity Chlamydomonas reinhardtii
4.1.1.39 T342I decrease in CO2/O2 specificity Chlamydomonas reinhardtii
4.1.1.39 V331A altered CO2/O2 specificity Chlamydomonas reinhardtii
4.1.1.39 V331A/G344S altered CO2/O2 specificity Chlamydomonas reinhardtii
4.1.1.39 V331A/T342I altered CO2/O2 specificity Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Chlamydomonas reinhardtii
-
-
-
4.1.1.39 Chlamydomonas reinhardtii 2137 mt+
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 Superdex-200 Hiload 16/60 gel filtration and MonoQ anion exchange column chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Chlamydomonas reinhardtii 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Chlamydomonas reinhardtii 2137 mt+ 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + O2
-
 Chlamydomonas reinhardtii 3-phospho-D-glycerate + 2-phosphoglycolate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + O2
-
 Chlamydomonas reinhardtii 2137 mt+ 3-phospho-D-glycerate + 2-phosphoglycolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 ribulose-1,5-bisphosphate carboxylase/oxygenase
-
 Chlamydomonas reinhardtii
4.1.1.39 Rubisco
-
 Chlamydomonas reinhardtii