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Literature summary extracted from

  • Campos-Bermudez, V.A.; Leite, N.R.; Krog, R.; Costa-Filho, A.J.; Soncini, F.C.; Oliva, G.; Vila, A.J.
    Biochemical and structural characterization of Salmonella typhimurium glyoxalase II: new insights into metal ion selectivity (2007), Biochemistry, 46, 11069-11079.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.6 hanging-drop vapor diffusion method at 4°C and 18°C, the crystal structure of GloB solved at 1.4 A Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.241
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 0.295
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Zn2+ Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 0.408
-
 S-D-lactoylglutathione enzyme otained from LB medium Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 0.463
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Fe2+ Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+ proteins expressed in minimal medium supplemented with Zn2+, Mn2+ or Fe2+ show similar activities Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 Mn2+ proteins expressed in minimal medium supplemented with Zn2+, Mn2+ or Fe2+ show similar activities Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 Zn2+ proteins expressed in minimal medium supplemented with Zn2+, Mn2+ or Fe2+ show similar activities Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZRM2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione
-
 Salmonella enterica subsp. enterica serovar Typhimurium D-lactate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 GloB
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 glyoxalase II
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 16
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Fe2+ Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 26.7
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Zn2+ Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 149.9
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Fe2+ Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 168.8
-
 S-D-lactoylglutathione enzyme otained from LB medium Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 209.9
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Zn2+ Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 394.9
-
 S-D-lactoylglutathione enzyme obtained from minimal medium supplemented with Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium