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Literature summary extracted from

  • Yu, L.; Blaser, M.; Andrei, P.I.; Pierik, A.J.; Selmer, T.
    4-hydroxyphenylacetate decarboxylases: Properties of a novel subclass of glycyl radical enzyme systems (2006), Biochemistry, 45, 9584-9592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.83 expressed in Escherichia coli Rosetta (DE3) pLysS cells Clostridioides difficile
4.1.1.83 expressed in Escherichia coli Rosetta (DE3) pLysS cells Clostridium scatologenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.83 0.358
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes
4.1.1.83 0.388
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes
4.1.1.83 0.41
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile
4.1.1.83 0.649
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.83 Fe 28-32 iron atoms per native molecule Clostridioides difficile
4.1.1.83 Fe 30-32 iron atoms per native molecule Clostridium scatologenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.83 440000
-
 SDS-PAGE Clostridium scatologenes
4.1.1.83 445000
-
 SDS-PAGE Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.83 Clostridioides difficile
-
-
-
4.1.1.83 Clostridium scatologenes
-
-
-
4.1.1.83 Clostridium scatologenes 957
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.83 Streptactin-Macroprep column chromatography Clostridioides difficile
4.1.1.83 Streptactin-Macroprep column chromatography Clostridium scatologenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.83 (3,4-dihydroxyphenyl)acetate + H+
-
 Clostridioides difficile 4-methylcatechol + CO2
-
 ?
4.1.1.83 (3,4-dihydroxyphenyl)acetate + H+
-
 Clostridium scatologenes 4-methylcatechol + CO2
-
 ?
4.1.1.83 (3,4-dihydroxyphenyl)acetate + H+
-
 Clostridium scatologenes 957 4-methylcatechol + CO2
-
 ?
4.1.1.83 (4-hydroxyphenyl)acetate + H+ (4-hydroxyphenyl)acetate is p-cresol Clostridioides difficile 4-methylphenol + CO2
-
 ?
4.1.1.83 (4-hydroxyphenyl)acetate + H+ (4-hydroxyphenyl)acetate is p-cresol Clostridium scatologenes 4-methylphenol + CO2
-
 ?
4.1.1.83 (4-hydroxyphenyl)acetate + H+ (4-hydroxyphenyl)acetate is p-cresol Clostridium scatologenes 957 4-methylphenol + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.83 heterooctamer
-
 Clostridium scatologenes

Synonyms

EC Number Synonyms Comment Organism
4.1.1.83 4-hydroxyphenylacetate decarboxylase
-
 Clostridioides difficile
4.1.1.83 4-hydroxyphenylacetate decarboxylase
-
 Clostridium scatologenes
4.1.1.83 CSD
-
 Clostridium scatologenes
4.1.1.83 HPA decarboxylase
-
 Clostridioides difficile
4.1.1.83 HPA decarboxylase
-
 Clostridium scatologenes
4.1.1.83 HPD
-
 Clostridioides difficile

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.83 65
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile
4.1.1.83 67
-
 3,4-Dihydroxyphenylacetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes
4.1.1.83 110
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridioides difficile
4.1.1.83 135
-
 (4-hydroxyphenyl)acetate in 100 mM Tris-HCl, pH 7.5, 40 mM NaCl, 5 mM MgCl2, 5 mM (NH4)2SO4, 5 mM cysteine, 25 mM substrate, and 5 mM dithiothreitol, at 30°C Clostridium scatologenes