EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.2 | 0.086 | - |
(R)-2-methylmalonyl-CoA | 37°C | Methylorubrum extorquens | |
5.4.99.2 | 0.152 | - |
(R)-2-methylmalonyl-CoA | presence of P-loop GTPase MeaB and GDP, 37°C | Methylorubrum extorquens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.2 | Methylorubrum extorquens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Methylorubrum extorquens | succinyl-CoA | - |
? | |
5.4.99.2 | additional information | P-loop GTPase MeaB plays an auxiliary role in catalytic reaction. MeaB increases affinity of the mutase for cofactor 5-deoxyadenosylcobalamin twofold | Methylorubrum extorquens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.2 | More | P-loop GTPase MeaB functions in GTP-dependent assembly of holomethyl-malonyl-CoA mutase and subsequent protection of radical intermediates during catalysis | Methylorubrum extorquens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.2 | 132 | - |
(R)-2-methylmalonyl-CoA | 37°C | Methylorubrum extorquens | |
5.4.99.2 | 237 | - |
(R)-2-methylmalonyl-CoA | presence of P-loop GTPase MeaB and GDP, 37°C | Methylorubrum extorquens | |
5.4.99.2 | 255 | - |
(R)-2-methylmalonyl-CoA | presence of P-loop GTPase MeaB, 37°C | Methylorubrum extorquens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.2 | 5'-deoxyadenosylcobalamin | P-loop GTPase MeaB increases affinity of the mutase for cofactor twofold, in presence of MeaB and GDP, affinity decreases fivifold | Methylorubrum extorquens |