BRENDA - Enzyme Database show

Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors

Frere, F.; Nentwich, M.; Gacond, S.; Heinz, D.W.; Neier, R.; Frankenberg-Dinkel, N.; Biochemistry 45, 8243-8253 (2006)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.24
hanging drop vapor-diffusion method. Crystal structures of the active site of Pseudomonas aeruginosa PBGS with the various inhibitors 5-hydroxylevulinic acid, 5,5'-oxybis(4-oxopentanoic acid), 5,5'-iminobis(4-oxopentanoic acid), 5,5'-thiobis(4-oxopentanoic acid), 5,5'-sulfinylbis(4-oxopentanoic acid) or 5,5'-sulfonylbis(4-oxopentanoic acid)
Pseudomonas aeruginosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5,5'-iminobis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-oxybis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-sulfinylbis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-sulfonylbis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-thiobis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5-hydroxylevulinic acid
-
Pseudomonas aeruginosa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Mg2+
enzyme contains Mg2+ in the active site
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
Pseudomonas aeruginosa
porphobilinogen synthase catalyzes the first committed step of the tetrapyrrole biosynthesis pathway
porphobilinogen + 2 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Pseudomonas aeruginosa
Q59643
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
porphobilinogen synthase catalyzes the first committed step of the tetrapyrrole biosynthesis pathway
678204
Pseudomonas aeruginosa
porphobilinogen + 2 H2O
-
-
-
?
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
enzymatic mechanism starts with formation of a C-C bond, linking C3 of the A-side 5-aminolevulinic acid to C4 of the P-side 5-aminolevulinic acid through an aldole addition
678204
Pseudomonas aeruginosa
porphobilinogen + 2 H2O
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.24
0.313
-
5,5'-iminobis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
0.342
-
5,5'-sulfonylbis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
0.963
-
5,5'-oxybis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
4.05
-
5-hydroxylevulinic acid
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
9.94
-
5,5'-sulfinylbis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
38.4
-
5,5'-thiobis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.24
hanging drop vapor-diffusion method. Crystal structures of the active site of Pseudomonas aeruginosa PBGS with the various inhibitors 5-hydroxylevulinic acid, 5,5'-oxybis(4-oxopentanoic acid), 5,5'-iminobis(4-oxopentanoic acid), 5,5'-thiobis(4-oxopentanoic acid), 5,5'-sulfinylbis(4-oxopentanoic acid) or 5,5'-sulfonylbis(4-oxopentanoic acid)
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5,5'-iminobis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-oxybis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-sulfinylbis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-sulfonylbis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5,5'-thiobis(4-oxopentanoic acid)
-
Pseudomonas aeruginosa
4.2.1.24
5-hydroxylevulinic acid
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.24
0.313
-
5,5'-iminobis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
0.342
-
5,5'-sulfonylbis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
0.963
-
5,5'-oxybis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
4.05
-
5-hydroxylevulinic acid
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
9.94
-
5,5'-sulfinylbis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
4.2.1.24
38.4
-
5,5'-thiobis(4-oxopentanoic acid)
37°C, pH 8.5
Pseudomonas aeruginosa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Mg2+
enzyme contains Mg2+ in the active site
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
Pseudomonas aeruginosa
porphobilinogen synthase catalyzes the first committed step of the tetrapyrrole biosynthesis pathway
porphobilinogen + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
porphobilinogen synthase catalyzes the first committed step of the tetrapyrrole biosynthesis pathway
678204
Pseudomonas aeruginosa
porphobilinogen + 2 H2O
-
-
-
?
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
enzymatic mechanism starts with formation of a C-C bond, linking C3 of the A-side 5-aminolevulinic acid to C4 of the P-side 5-aminolevulinic acid through an aldole addition
678204
Pseudomonas aeruginosa
porphobilinogen + 2 H2O
-
-
-
?