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Literature summary extracted from

  • Granata, V.; Graziano, G.; Ruggiero, A.; Raimo, G.; Masullo, M.; Arcari, P.; Vitagliano, L.; Zagari, A.
    Chemical denaturation of the elongation factor 1alpha isolated from the hyperthermophilic archaeon Sulfolobus solfataricus (2006), Biochemistry, 45, 719-726.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.3 recombinant SsEF-1alpha and its nucleotide-free form are prepared by using an Escherichia coli expression system Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.5.3 guanidine hydrochloride deactivation by denaturation of the protein Saccharolobus solfataricus
3.6.5.3 Urea deactivation by denaturation of the protein Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.3 GTP + H2O Saccharolobus solfataricus
-
 GDP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Saccharolobus solfataricus
-
-
-
3.6.5.3 Saccharolobus solfataricus
-
 elongation factor 1alpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.3
-
 Saccharolobus solfataricus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.6.5.3 denaturation by urea and guanidine hydrochloride shows a cooperative unfolding process with no intermediate species. Chemical unfolding by urea and guanidine hydrochloride is fully reversible for both enzyme-GDP complex and nucleotide-free enzyme. Both forms exhibit remarkable stability against urea, but not against guanidine hydrochloride Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
3.6.5.3 -20°C, 20 mM Tris/HCl, pH 7.8, 50 mM KCl, 10 mM MgCl2, 50% v/v glycerol Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.6.5.3 elongation factor 1alpha
-
 Saccharolobus solfataricus
3.6.5.3 protein-synthesizing GTPase
-
 Saccharolobus solfataricus
3.6.5.3 SsEF-1alpha
-
 Saccharolobus solfataricus