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Literature summary extracted from

  • Arent, S.; Kadziola, A.; Larsen, S.; Neuhard, J.; Jensen, K.F.
    The extraordinary specificity of xanthine phosphoribosyltransferase from Bacillus subtilis elucidated by reaction kinetics, ligand binding, and crystallography (2006), Biochemistry, 45, 6615-6627.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.22 crystal structure of the dimeric XPRTase-GMP complex was determined to 2.05 A resolution Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.22 GMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate Bacillus subtilis
2.4.2.22 Pyrophosphate noncompetitive Bacillus subtilis
2.4.2.22 XMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.22 0.0022
-
 xanthine
-
 Bacillus subtilis
2.4.2.22 0.03
-
 5-phospho-alpha-D-ribose 1-diphosphate cosubstrate: xanthine Bacillus subtilis
2.4.2.22 0.281
-
 guanine
-
 Bacillus subtilis
2.4.2.22 1.25
-
 hypoxanthine
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.22 Bacillus subtilis P42085
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.22 purification and formation of nucleotide free, GMP and XMP saturated complexes Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + guanine
-
 Bacillus subtilis GMP + diphosphate
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine
-
 Bacillus subtilis IMP + diphosphate
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + xanthine in a sequential reaction mechanism XPRTase binds first 5-phospho-alpha-D-ribose 1-diphosphate, stabilizing its active dimeric form, and subsequently xanthine Bacillus subtilis 9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.22 XPRTase
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.22 0.03
-
 guanine
-
 Bacillus subtilis
2.4.2.22 0.5
-
 hypoxanthine
-
 Bacillus subtilis
2.4.2.22 11
-
 xanthine
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.22 8 8.5 substrate: xanthine Bacillus subtilis
2.4.2.22 9.5
-
 substrate: hypoxanthine Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.22 0.022
-
 XMP Kis Bacillus subtilis
2.4.2.22 0.033
-
 GMP Kis Bacillus subtilis