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Literature summary extracted from

  • Sengupta, S.; Horowitz, P.M.; Karsten, S.L.; Jackson, G.R.; Geschwind, D.H.; Fu, Y.; Berry, R.W.; Binder, L.I.
    Degradation of tau protein by puromycin-sensitive aminopeptidase in vitro (2006), Biochemistry, 45, 15111-15119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.14 expressed in Escherichia coli BL-21 (DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.14 anti-PSA immunglobulinb G 53.4% residual activity at 0.1 mg/ml Homo sapiens
3.4.11.14 Aprotinin 82% residual activity at 0.1 mM Homo sapiens
3.4.11.14 bestatin 2.57% residual activity at 0.1 mM Homo sapiens
3.4.11.14 EDTA 44% residual activity at 0.5 mM Homo sapiens
3.4.11.14 leupeptin 83% residual activity at 0.1 mM Homo sapiens
3.4.11.14 additional information not inhibited by phenylmethylsulfonylfluoride Homo sapiens
3.4.11.14 puromycin 3.12% residual activity at 0.1 mM Homo sapiens
3.4.11.14 Zn2+ 79.4% residual activity at 0.01 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.14 0.21
-
 Val-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 0.25
-
 Leu-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 0.27
-
 Ala-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 0.47
-
 Pro-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 0.8
-
 Met-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 2.2
-
 Lys-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.14 Ca2+ 9.8% increase of activity at 5 mM Homo sapiens
3.4.11.14 Mg2+ 26% increase of activity at 1 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.14 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.14 glutathione Sepharose column chromatography and Superdex-200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.14 Ala-p-nitroanilide + H2O
-
 Homo sapiens Ala + p-nitroaniline
-
 ?
3.4.11.14 Leu-p-nitroanilide + H2O
-
 Homo sapiens Leu + p-nitroaniline
-
 ?
3.4.11.14 Lys-p-nitroanilide + H2O
-
 Homo sapiens Lys + p-nitroaniline
-
 ?
3.4.11.14 Met-p-nitroanilide + H2O
-
 Homo sapiens Met + p-nitroaniline
-
 ?
3.4.11.14 Pro-p-nitroanilide + H2O
-
 Homo sapiens Pro + p-nitroaniline
-
 ?
3.4.11.14 Val-p-nitroanilide + H2O
-
 Homo sapiens Val + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.14 PSA
-
 Homo sapiens
3.4.11.14 puromycin-sensitive aminopeptidase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.14 1.43
-
 Val-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 3.5
-
 Leu-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 3.8
-
 Ala-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 16.9
-
 Pro-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 19.83
-
 Leu-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 20.83
-
 Ala-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 23.17
-
 Pro-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 30
-
 Met-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.4.11.14 89
-
 Lys-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens