EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.81 | expression in Escherichia coli strain BL21 | Bradyrhizobium japonicum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.1.81 | all crystals, wild type TarD liganded with Mg2+, selenomethionine-substituted TarD liganded with Mg2+, wild-type TarD liganded with Mg2+ and meso-tartrate, and the K184A mutant liganded with Mg2+ and D-tartrate, are grown by the hanging drop method at room temperature | Bradyrhizobium japonicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.81 | H322A | kcatKm is 193000fold lower than wild-type value | Bradyrhizobium japonicum |
4.2.1.81 | H322N | kcatKm is 607fold lower than wild-type value | Bradyrhizobium japonicum |
4.2.1.81 | H322Q | kcatKm is 340000fold lower than wild-type value | Bradyrhizobium japonicum |
4.2.1.81 | K102A | inactive mutant protein | Bradyrhizobium japonicum |
4.2.1.81 | K102M | inactive mutant protein | Bradyrhizobium japonicum |
4.2.1.81 | K184A | inactive mutant enzyme | Bradyrhizobium japonicum |
4.2.1.81 | K184R | reduced activity. kcat/Km is 500fold lower than wild-type value | Bradyrhizobium japonicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.81 | meso-tartrate | competitive inhibitor | Bradyrhizobium japonicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.81 | 0.035 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme K184R | Bradyrhizobium japonicum | |
4.2.1.81 | 0.048 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme H322N | Bradyrhizobium japonicum | |
4.2.1.81 | 0.086 | - |
(S,S)-Tartrate | 25°C, pH 7.5, wild-type enzyme | Bradyrhizobium japonicum | |
4.2.1.81 | 6.2 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme H322A | Bradyrhizobium japonicum | |
4.2.1.81 | 7.7 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme H322Q | Bradyrhizobium japonicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.81 | Bradyrhizobium japonicum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.81 | - |
Bradyrhizobium japonicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.81 | (S,S)-tartrate | mechanism in which Lys 184 initiates the reaction by abstraction of the R-proton to generate a Mg2+-stabilized enediolate intermediate, and the vinylogous beta-elimination of the 3-OH group is general acid-catalyzed by the His 322, accomplishing the anti-elimination of water. The replacement of the leaving group by solvent-derived hydrogen is stereorandom | Bradyrhizobium japonicum | oxaloacetate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.81 | bll6730 | - |
Bradyrhizobium japonicum |
4.2.1.81 | TarD | - |
Bradyrhizobium japonicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.81 | 0.0019 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme H322Q | Bradyrhizobium japonicum | |
4.2.1.81 | 0.0027 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme H322A | Bradyrhizobium japonicum | |
4.2.1.81 | 0.0058 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme K184R | Bradyrhizobium japonicum | |
4.2.1.81 | 0.0069 | - |
(S,S)-Tartrate | 25°C, pH 7.5, mutant enzyme H322N | Bradyrhizobium japonicum | |
4.2.1.81 | 7.3 | - |
(S,S)-Tartrate | 25°C, pH 7.5, wild-type enzyme | Bradyrhizobium japonicum |