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Literature summary extracted from
- Deuterium isotope effects in the unusual addition of toluene to fumarate catalyzed by benzylsuccinate synthase (2006), Biochemistry, 45, 13932-13938.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.11 | 98000 | - |
98000, SDS-PAGE of the alpha subunit | Thauera aromatica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.11 | toluene + fumarate | Thauera aromatica | first step in the anaerobic degradation pathway of toluene | benzylsuccinate | - |
ir |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.11 | Thauera aromatica | - |
grown with toluene as carbon source | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.1.99.11 | extremely oxygen sensitive | Thauera aromatica |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.11 | loss of activity during purification due to high oxygen sensitivity | Thauera aromatica |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.99.11 | benzylsuccinate = toluene + fumarate | mechanism | Thauera aromatica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.11 | 0.0024 | - |
cell extract, 4°C | Thauera aromatica |
| 4.1.99.11 | 0.0074 | - |
cell extract, 30°C | Thauera aromatica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.11 | toluene + fumarate | first step in the anaerobic degradation pathway of toluene | Thauera aromatica | benzylsuccinate | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.99.11 | ? | 98000, SDS-PAGE of the alpha subunit | Thauera aromatica |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.11 | 0.11 | - |
Toluene | cell extract, 4°C | Thauera aromatica | |
| 4.1.99.11 | 0.35 | - |
Toluene | cell extract, 30°C | Thauera aromatica | |
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Release 2023.1 (January 2023)
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