EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.9 | expression in Escherichia coli | Escherichia coli |
3.2.2.9 | expression in Escherichia coli | Streptococcus pneumoniae |
3.2.2.16 | expression in Escherichia coli | Escherichia coli |
3.2.2.16 | expression in Escherichia coli | Streptococcus pneumoniae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.2.9 | enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket | Escherichia coli |
3.2.2.9 | enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket | Streptococcus pneumoniae |
3.2.2.16 | enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket | Escherichia coli |
3.2.2.16 | enzyme in complex with transition state analogue methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A. Enzyme forms a dimer with the methylthio group in a flexible hydrophobic pocket | Streptococcus pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.9 | 4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
3.2.2.9 | 4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.9 | ethylthio-immucillin-A | - |
Escherichia coli | |
3.2.2.9 | ethylthio-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.9 | methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
3.2.2.9 | methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.9 | methylthio-immucillin-A | weak | Escherichia coli | |
3.2.2.9 | methylthio-immucillin-A | weak | Streptococcus pneumoniae | |
3.2.2.9 | p-tolylthio-immucillin-A | - |
Escherichia coli | |
3.2.2.9 | p-tolylthio-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.9 | phenylthio-immucillin-A | - |
Escherichia coli | |
3.2.2.9 | phenylthio-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.16 | 4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
3.2.2.16 | 4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.16 | ethylthio-immucillin-A | - |
Escherichia coli | |
3.2.2.16 | ethylthio-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.16 | methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Escherichia coli | |
3.2.2.16 | methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.16 | methylthio-immucillin-A | weak | Escherichia coli | |
3.2.2.16 | methylthio-immucillin-A | weak | Streptococcus pneumoniae | |
3.2.2.16 | p-tolylthio-immucillin-A | - |
Escherichia coli | |
3.2.2.16 | p-tolylthio-immucillin-A | - |
Streptococcus pneumoniae | |
3.2.2.16 | phenylthio-immucillin-A | - |
Escherichia coli | |
3.2.2.16 | phenylthio-immucillin-A | - |
Streptococcus pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.013 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.023 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.43 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 1.3 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.013 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.023 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.43 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 1.3 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.9 | Escherichia coli | - |
bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
3.2.2.9 | Streptococcus pneumoniae | - |
bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
3.2.2.16 | Escherichia coli | - |
bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
3.2.2.16 | Streptococcus pneumoniae | Q8DQ16 | bifunctional 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | 5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.9 | 5'-methylthioadenosine + H2O | - |
Streptococcus pneumoniae | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.9 | additional information | no substrate: adenosine, inosine | Escherichia coli | ? | - |
? | |
3.2.2.9 | additional information | no substrate: adenosine, inosine | Streptococcus pneumoniae | ? | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Streptococcus pneumoniae | adenine + S-ribosyl-L-homocysteine | - |
? | |
3.2.2.16 | 5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.16 | 5'-methylthioadenosine + H2O | - |
Streptococcus pneumoniae | 5-methylthio-D-ribose + adenine | - |
? | |
3.2.2.16 | S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
3.2.2.16 | S-adenosyl-L-homocysteine + H2O | - |
Streptococcus pneumoniae | adenine + S-ribosyl-L-homocysteine | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.25 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.37 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 2.1 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 4 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.25 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.37 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 2.1 | - |
S-adenosyl-L-homocysteine | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 4 | - |
5'-methylthioadenosine | pH 7.5, 25°C | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.00000036 | - |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 0.00000036 | - |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.000024 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 0.000024 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.00004 | - |
ethylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 0.00004 | - |
ethylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.00006 | - |
p-tolylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 0.00006 | - |
p-tolylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.000335 | - |
phenylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 0.000335 | - |
phenylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.9 | 0.001 | - |
methylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.9 | 0.001 | - |
methylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.00000036 | - |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.00000036 | - |
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.000024 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.000024 | - |
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.00004 | - |
ethylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.00004 | - |
ethylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.00006 | - |
p-tolylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.00006 | - |
p-tolylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.000335 | - |
phenylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.000335 | - |
phenylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae | |
3.2.2.16 | 0.001 | - |
methylthio-immucillin-A | pH 7.5, 25°C | Escherichia coli | |
3.2.2.16 | 0.001 | - |
methylthio-immucillin-A | pH 7.5, 25°C | Streptococcus pneumoniae |