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Literature summary extracted from

  • Nemec, K.N.; Pande, A.H.; Qin, S.; Bieber Urbauer, R.J.; Tan, S.; Moe, D.; Tatulian, S.A.
    Structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2 (2006), Biochemistry, 45, 12448-12460.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 F5W site-directed mutagenesis, mutation of the substrate binding pocket leads to reduced enzyme activity, the inhibitory effect of the F5W mutation results from the high affinity of Trp for the membrane-water interface Homo sapiens
3.1.1.4 V3W site-directed mutagenesis, mutation of the membrane binding surface residue results in substantial enhancement of the enzyme activity, the stimulatory effect of the V3W mutation results from the high affinity of Trp for the membrane-water interface Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 additional information
-
 additional information kinetics and membrane binding of recombinant wild-type and mutant enzymes, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 extracellular secretory phospholipase A2 Homo sapiens
-
-
3.1.1.4 membrane hIBPLA2, structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, overview Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 recombinant solubilized wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography and gel filtration to homogeneity Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.4 recombinant wild-type and mutant enzymes from Escherichia coli inclusion bodies Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-bis-(10-pyrenedecanoyl)-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-(10-pyrenedecanoyl)-glycero-3-phosphocholine + 10-pyrenedecanoate
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Homo sapiens 1-palmitoyl-glycero-3-phosphoglycerol + palmitate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphorylglycerol + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-stearoyl-dibromo-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.4 additional information structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 hIIAPLA2
-
 Homo sapiens
3.1.1.4 human group IIA phospholipase A2
-
 Homo sapiens
3.1.1.4 PLA2
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 7.5
-
 assay at Homo sapiens