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Literature summary extracted from

  • Pande, A.H.; Qin, S.; Nemec, K.N.; He, X.; Tatulian, S.A.
    Isoform-specific membrane insertion of secretory phospholipase A2 and functional implications (2006), Biochemistry, 45, 12436-12447.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 additional information isozyme-specific membrane insertion of secretory phospholipase A2 is controlled by membrane fluidity and is necessary for the full activity of the enzyme, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 expression of isozyme hIBPLA2 and wild-type and mutant isozymes hIIAPLA2 in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 V3W site-directed mutagenesis, mutant of human group IIA PLA2, structure comparison to isozyme human group IB PLA2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 additional information
-
 additional information kinetics of recombinant wild-type and mutant enzymes Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 extracellular
-
 Apis mellifera
-
-
3.1.1.4 extracellular secretory phospholipase A2 Homo sapiens
-
-
3.1.1.4 membrane isozyme-specific membrane insertion of secretory phospholipase A2 is controlled by membrane fluidity and is necessary for the full activity of the enzyme, membrane anionic surface charge facilitates membrane Recruitment and ActiVity of hIBPLA2, overview Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Apis mellifera P00630
-
-
3.1.1.4 Homo sapiens P14555
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 native enzyme from venom by gel filtration to homogeneity Apis mellifera
3.1.1.4 recombinant human group IIA PLA2isozyme and isozyme hIBPLA2 from Escherichia coli strain BL21(DE3) Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 venom
-
 Apis mellifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-bis-(10-pyrenedecanoyl)-sn-glycero-3-phosphocholine + H2O
-
 Apis mellifera 1-(10-pyrenedecanoyl)-glycero-3-phosphocholine + 10-pyrenedecanoate
-
 ?
3.1.1.4 1,2-bis-(10-pyrenedecanoyl)-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-(10-pyrenedecanoyl)-glycero-3-phosphocholine + 10-pyrenedecanoate
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Apis mellifera 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Homo sapiens 1-palmitoyl-glycero-3-phosphoglycerol + palmitate
-
 ?
3.1.1.4 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Apis mellifera 1-palmitoyl-sn-glycero-3-phosphorylglycerol + palmitate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
-
 Apis mellifera 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Apis mellifera 1-palmitoyl-sn-glycero-3-phosphorylglycerol + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphorylglycerol + oleate
-
 ?
3.1.1.4 1-palmitoyl-2-stearoyl-dibromo-sn-glycero-3-phosphocholine + H2O
-
 Apis mellifera ?
-
 ?
3.1.1.4 1-palmitoyl-2-stearoyl-dibromo-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 hIBPLA2
-
 Homo sapiens
3.1.1.4 hIIAPLA2
-
 Homo sapiens
3.1.1.4 human group IB PLA2
-
 Homo sapiens
3.1.1.4 PLA2
-
 Apis mellifera
3.1.1.4 secretory phospholipase A2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 25
-
 assay at Apis mellifera
3.1.1.4 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 7.4
-
 assay at Apis mellifera
3.1.1.4 7.4
-
 assay at Homo sapiens