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Literature summary extracted from

  • Raia, C.A.; Caruso, C.; Marino, M.; Vespa, N.; Rossi, M.
    Activation of Sulfolobus solfataricus alcohol dehydrogenase by modification of cysteine residue 38 with iodoacetic acid (1996), Biochemistry, 35, 638-647.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 sodium iodoacetate increasing concentrations od sodium iodoacetate produce a slight decrease in activity Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.03
-
 NADH native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.04
-
 NADH carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.045
-
 Cyclohexanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.12
-
 4-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.14
-
 4-methoxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.14
-
 3-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.2
-
 benzaldehyde carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.2
-
 NAD+ native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.23
-
 Cyclohexanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.28
-
 4-carboxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.3
-
 benzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.33
-
 1-propanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.4
-
 4-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.5
-
 NAD+ carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.6
-
 2-propanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.7
-
 ethanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.8
-
 benzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.9
-
 3-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.2
-
 3-Methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.3
-
 4-methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.4
-
 4-methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 2
-
 benzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 2.4
-
 1-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 3.3
-
 3-Methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 53
-
 2-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 184
-
 ethanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharolobus solfataricus
-
 strain DSM1617
-
1.1.1.1 Saccharolobus solfataricus DSM 1617
-
 strain DSM1617
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.1 additional information carboxymethylation with sodium iodoacetate Saccharolobus solfataricus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 Blue A column chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-propanol + NAD+
-
 Saccharolobus solfataricus propanal + NADH + H+
-
 ?
1.1.1.1 1-propanol + NAD+
-
 Saccharolobus solfataricus DSM 1617 propanal + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Saccharolobus solfataricus 2-propanone + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Saccharolobus solfataricus DSM 1617 2-propanone + NADH + H+
-
 ?
1.1.1.1 3-bromobenzyl alcohol + NAD+
-
 Saccharolobus solfataricus 3-bromobenzaldehyde + NADH + H+
-
 ?
1.1.1.1 3-methoxybenzyl alcohol + NAD+
-
 Saccharolobus solfataricus 3-methoxybenzaldehyde + NADH + H+
-
 ?
1.1.1.1 4-bromobenzyl alcohol + NAD+
-
 Saccharolobus solfataricus 4-bromobenzaldehyde + NADH + H+
-
 ?
1.1.1.1 4-carboxybenzaldehyde + NADH + H+
-
 Saccharolobus solfataricus 4-carboxybenzyl alcohol + NAD+
-
 ?
1.1.1.1 4-methoxybenzyl alcohol + NAD+
-
 Saccharolobus solfataricus 4-methoxybenzaldehyde + NADH + H+
-
 r
1.1.1.1 4-nitrobenzaldehyde + NADH + H+
-
 Saccharolobus solfataricus 4-nitrobenzyl alcohol + NAD+
-
 ?
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH + H+
-
 r
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharolobus solfataricus DSM 1617 benzaldehyde + NADH + H+
-
 r
1.1.1.1 cyclohexanol + NAD+
-
 Saccharolobus solfataricus cyclohexanone + NADH + H+
-
 ?
1.1.1.1 cyclohexanol + NAD+
-
 Saccharolobus solfataricus DSM 1617 cyclohexanone + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Saccharolobus solfataricus acetaldehyde + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Saccharolobus solfataricus DSM 1617 acetaldehyde + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 55
-
 the native enzyme remains completely stable after heating for 3 h at 55°C while the carboxymethylated enzyme loses 10% of activity under the same conditions Saccharolobus solfataricus
1.1.1.1 65
-
 at 65°C the half-lives of the native and carboxymethylated enzymes are 9 h and 4 h, respectively Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.25
-
 2-propanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.45
-
 2-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.5
-
 ethanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.6
-
 4-methoxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.65
-
 Cyclohexanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.7
-
 4-carboxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 0.85
-
 ethanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.1
-
 4-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.2
-
 Cyclohexanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.2
-
 1-propanol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.2
-
 3-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.4
-
 NAD+ native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.4
-
 benzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.4
-
 3-Methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.5
-
 4-methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 1.8
-
 benzaldehyde carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 2
-
 NADH carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 2
-
 3-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 2.2
-
 1-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 3.5
-
 4-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 3.5
-
 benzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 4.5
-
 NADH native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 4.6
-
 4-nitrobenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 14.5
-
 benzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 16
-
 3-Methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 18.5
-
 NAD+ carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1.1.1 20.5
-
 4-methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 8.5 9.1
-
 Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ dependent Saccharolobus solfataricus