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Literature summary extracted from

  • Master, E.R.; Zheng, Y.; Storms, R.; Tsang, A.; Powlowski, J.
    A xyloglucan-specific family 12 glycosyl hydrolase from Aspergillus niger: recombinant expression, purification and characterization (2008), Biochem. J., 411, 161-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 expression in Aspergillus niger Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information Km value for xyloglucan is 0.39 mg/ml at 50°C, pH 5.0 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.151 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 23300
-
 x * 23300, calculated, mature protein, x * 32000, SDS-PAGE Aspergillus niger
3.2.1.151 32000
-
 x * 23300, calculated, mature protein, x * 32000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Aspergillus niger A1XP58
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.151 no glycoprotein N-glycosylation site at N32 is not glycosylated Aspergillus niger
3.2.1.151 proteolytic modification sequence contains a signal peptide of 15 amino acids Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.151 culture fluid
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.151 83
-
 50°C, pH 5.0 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 carboxymethyl curdlan + H2O
-
 Aspergillus niger carboxymethyl curdlan oligosaccharides less than 2% of the activity with xyloglucan ?
3.2.1.151 carboxymethyl pachyman + H2O
-
 Aspergillus niger carboxymethyl pachyman oligosaccharides less than 2% of the activity with xyloglucan ?
3.2.1.151 additional information no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan Aspergillus niger ?
-
 ?
3.2.1.151 xyloglucan + H2O substrate xyloglucan from tamarind seed, cleavage at unbranched glucose in the backbone Aspergillus niger xyloglucan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 23300, calculated, mature protein, x * 32000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 50 60
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 34
-
 xyloglucan 50°C, pH 5.0 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5
-
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Aspergillus niger calculated
-
 3.6