Any feedback?
Literature summary extracted from
- A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae (2008), Biochem. J., 409, 399-406.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.1.18 | into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.18 | EDTA | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 10.2%, without 10.0% | Saccharomyces cerevisiae |  |
| 4.3.1.18 | hydroxylamine | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 64.4%, without 23.7% | Saccharomyces cerevisiae | |
| 4.3.1.18 | N-ethylmaleimide | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 19.5%, without 8.7% | Saccharomyces cerevisiae | |
| 4.3.1.18 | phenylhydrazin | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 97.1%, without 4.2% | Saccharomyces cerevisiae | |
| 4.3.1.18 | Sodium cyanoborohydride | 5 mM, DsdSC activity with pyridoxal 5'-phosphate 20.9%, without 12.1% | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.18 | 0.13 | - |
D-threonine | untreated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.17 | - |
D-threonine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.19 | - |
D-serine | EDTA-treated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.39 | - |
D-serine | untreated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.48 | - |
D-serine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.98 | - |
beta-Chloro-D-alanine | EDTA-treated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 1.45 | - |
beta-Chloro-D-alanine | untreated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 2.45 | - |
beta-Cl-D-alanine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.18 | Zn2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 50000 | - |
determined by SDS-PAGE | Saccharomyces cerevisiae |
| 4.3.1.18 | 50060 | - |
calculated molecular mass | Saccharomyces cerevisiae |
| 4.3.1.18 | 118000 | - |
dimer, determined by gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.18 | D-serine | Saccharomyces cerevisiae | - |
pyruvate + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.18 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.18 | using a Ni2+-chelating column and a DEAE-TOYOPEARL column | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.18 | beta-chloro-D-alanine | activity of approximately 2% | Saccharomyces cerevisiae | 3-chloro-D-alanine | - |
? | |
| 4.3.1.18 | D-serine | - |
Saccharomyces cerevisiae | pyruvate + NH3 | - |
? | |
| 4.3.1.18 | D-threonine | activity of approximately 3% | Saccharomyces cerevisiae | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.1.18 | dimer | - |
Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.1.18 | D-serine ammonia-lyase | - |
Saccharomyces cerevisiae |
| 4.3.1.18 | D-serine dehydratase | - |
Saccharomyces cerevisiae |
| 4.3.1.18 | DSD | - |
Saccharomyces cerevisiae |
| 4.3.1.18 | DsdSC | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 30 | - |
ammonia assay | Saccharomyces cerevisiae |
| 4.3.1.18 | 30 | - |
pyruvate assay | Saccharomyces cerevisiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.1.18 | 0.22 | - |
beta-Chloro-D-alanine | untreated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.24 | - |
beta-Chloro-D-alanine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.34 | - |
D-threonine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.43 | - |
D-threonine | untreated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 0.48 | - |
beta-Chloro-D-alanine | EDTA-treated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 1.67 | - |
D-serine | EDTA-treated | Saccharomyces cerevisiae | |
| 4.3.1.18 | 12.5 | - |
D-serine | EDTA-treated + 2.5 microM ZnCl2 | Saccharomyces cerevisiae | |
| 4.3.1.18 | 13.1 | - |
D-serine | untreated | Saccharomyces cerevisiae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 8 | - |
ammonia assay | Saccharomyces cerevisiae |
| 4.3.1.18 | 8 | - |
pyruvate assay | Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.18 | pyridoxal 5'-phosphate | PLP | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
