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Literature summary extracted from

  • Cotes, K.; Dhouib, R.; Douchet, I.; Chahinian, H.; de Caro, A.; Carriere, F.; Canaan, S.
    Characterization of an exported monoglyceride lipase from Mycobacterium tuberculosis possibly involved in the metabolism of host cell membrane lipids (2007), Biochem. J., 408, 417-427.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.23 taurodeoxycholate dependent on, maximal activity at 1-5 mM Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.23 gene Rv0183, DNA and amino acid sequence determination and anaylsis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.23 D226A site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis
3.1.1.23 D226N site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis
3.1.1.23 H256A site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis
3.1.1.23 S110A site-directed mutagenesis, active site residue mutation, the catalytic activity is affected Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.23 E600 inactivation Mycobacterium tuberculosis
3.1.1.23 additional information inactivation by serine esterase inhibitors Mycobacterium tuberculosis
3.1.1.23 PMSF inactivation Mycobacterium tuberculosis
3.1.1.23 tetrahydrolipstatin inactivation Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.23 additional information
-
 additional information kinetics Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.23 cell wall
-
 Mycobacterium tuberculosis 5618
-
3.1.1.23 extracellular
-
 Mycobacterium tuberculosis
-
-
3.1.1.23 additional information immunolocalization studies Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.23 30223
-
 x * 30223, detagged enzyme, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.23 additional information Mycobacterium tuberculosis Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids ?
-
 ?
3.1.1.23 additional information Mycobacterium tuberculosis H37Rv Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Mycobacterium tuberculosis
-
 gene Rv0183
-
3.1.1.23 Mycobacterium tuberculosis H37Rv
-
 gene Rv0183
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.23 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and gel filtration, removal of the His-tag Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.23 CnHmCO-C3O3H7 + H2O = CnHmCO2H + glycerol the catalytic triad is composed of Ser110, Asp226 and His256 residues Mycobacterium tuberculosis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.23 290
-
 purified recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 1,2-dicaprin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.1.1.23 1,2-dicaprin + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
3.1.1.23 1-mono-oleyl-rac-glycerol + H2O
-
 Mycobacterium tuberculosis oleate + glycerol
-
 ?
3.1.1.23 1-monobutyroyl-rac-glycerol + H2O
-
 Mycobacterium tuberculosis butyrate + glycerol
-
 ?
3.1.1.23 1-monodecanoyl-rac-glycerol + H2O
-
 Mycobacterium tuberculosis decanoate + glycerol
-
 ?
3.1.1.23 1-monolauroyl-rac-glycerol + H2O
-
 Mycobacterium tuberculosis laureate + glycerol
-
 ?
3.1.1.23 1-monomyristoyl-rac-glycerol + H2O
-
 Mycobacterium tuberculosis myristoate + glycerol
-
 ?
3.1.1.23 1-monooctanoyl-rac-glycerol + H2O
-
 Mycobacterium tuberculosis octanoate + glycerol
-
 ?
3.1.1.23 diolein + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.1.1.23 diolein + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
3.1.1.23 additional information Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids Mycobacterium tuberculosis ?
-
 ?
3.1.1.23 additional information substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview Mycobacterium tuberculosis ?
-
 ?
3.1.1.23 additional information Rv0183, as exported enzyme, may be involved in the degradation of the host cell lipids Mycobacterium tuberculosis H37Rv ?
-
 ?
3.1.1.23 additional information substrate specificity, the enzyme hydrolyses monoacylglycerol substrates, both long chain di- and triacylglycerols, although the turnover is lower than with monoacylglycerol, overview, no activity of the recombinant enzyme with lysophospholipid substrates, recombinant Rv0183 hydrolyses synthetic vinyl esters with various acyl chain lengths, chain length dependence, overview Mycobacterium tuberculosis H37Rv ?
-
 ?
3.1.1.23 soybean oil + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.1.1.23 soybean oil + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.23 ? x * 30223, detagged enzyme, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.23 monoglyceride lipase
-
 Mycobacterium tuberculosis
3.1.1.23 Rv0183 protein
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.23 37
-
-
 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.23 25 55
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 7.5 9
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.23 6 9 50% of maximal activity at pH 6.5, no activity below pH 6.0, maximal activity at pH 7.5-9.0 Mycobacterium tuberculosis