EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.4.53 | in complex with inhibitor 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid, comparison with isoforms PDE4A, PDE4B, PDE4C. Inhibitor binds in the same conformation to the deep cAMP substrate pocket and interacts with the same residues in each instance. Detailed structural comparison | Homo sapiens |
3.1.4.53 | in complex with inhibitor 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid, comparison with isoforms PDE4A, PDE4C, PDE4D. Inhibitor binds in the same conformation to the deep cAMP substrate pocket and interacts with the same resiudues in each instance. Detailed structural comparison | Homo sapiens |
3.1.4.53 | in complex with inhibitor 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid, comparison with isoforms PDE4B, PDE4C, PDE4D. Inhibitor binds in the same conformation to the deep cAMP substrate pocket and interacts with the same residues in each instance. Detailed structural comparison | Homo sapiens |
3.1.4.53 | unliganded, detailed structural comparison with isoforms PDE4A, PDE4B, PDE4C | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.53 | 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid | i.e. NVP; i.e. NVP; i.e. NVP | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.53 | 0.0008 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 0.0015 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 0.0045 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 0.0051 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 0.24 | - |
cGMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 0.427 | - |
cGMP | pH 7.5, 25°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.53 | Homo sapiens | - |
isoform PDE4B2B | - |
3.1.4.53 | Homo sapiens | - |
isoform PDE4C2 | - |
3.1.4.53 | Homo sapiens | P27815 | isoform PDE4A10 | - |
3.1.4.53 | Homo sapiens | Q08499 | isoform PDE4D2 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.53 | cAMP + H2O | - |
Homo sapiens | 5'-AMP | - |
? | |
3.1.4.53 | cGMP + H2O | - |
Homo sapiens | 5'-GMP | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.53 | 0.48 | - |
cGMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 0.66 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 1.19 | - |
cGMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 1.56 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 5.4 | - |
cAMP | pH 7.5, 25°C | Homo sapiens | |
3.1.4.53 | 6.7 | - |
cAMP | pH 7.5, 25°C | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.1.4.53 | 0.00057 | - |
pH 7.5, 25°C | Homo sapiens | 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid | |
3.1.4.53 | 0.00065 | - |
pH 7.5, 25°C | Homo sapiens | 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid | |
3.1.4.53 | 0.0033 | - |
pH 7.5, 25°C | Homo sapiens | 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid | |
3.1.4.53 | 0.0057 | - |
pH 7.5, 25°C | Homo sapiens | 4-[8-(3-nitrophenyl)-[1,7]naphthyridin-6-yl]benzoic acid |