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Literature summary extracted from

  • Wang, S.C.; Lai, H.L.; Chiu, Y.T.; Ou, R.; Huang, C.L.; Chern, Y.
    Regulation of type V adenylate cyclase by Ric8a, a guanine nucleotide exchange factor (2007), Biochem. J., 406, 383-388.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.1 forskolin
-
 Rattus norvegicus
4.6.1.1 isoprenaline
-
 Rattus norvegicus

Application

EC Number Application Comment Organism
4.6.1.1 additional information interaction between the N-terminus of AC5 and the guanine nucleotide exchange factor Ric8a provides a pathway to fine-tune AC5 activity via a Galphai mediated pathway Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 Escherichia coli BL21 (DE3) transformed with pET11D-AC5N1-215, AC5-myc and HA-Ric8a expressed in HEK-293T cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.1 Galphai2G203T chronic treatment of HEK-293T cells reduces AC5 activity Rattus norvegicus
4.6.1.1 pertussis toxin chronic treatment of HEK-293T cells reduces AC5 activity Rattus norvegicus
4.6.1.1 Ric8a i.e., resistance to inhibitors of cholinesterase 8 homolog A, AC5 interacts with Ric8a through directly interacting at its N-terminus, selectively suppresses AC5 activity, does not further suppress AC5’s activity in the presence of somatostatin, effectively suppresses the forskolin-stimulated activity of AC5, but not that of AC6. Treating cells with pertussis toxin or expressing a dominant negative Galphai mutant abolishes the suppressive effect, markedly suppresses the activation of AC5 induced by isoprenaline of a Galphas coupled receptor in an isoform-specific manner, while the isoprenaline-evoked activity of AC6 is not affected Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 membrane
-
 Rattus norvegicus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Rattus norvegicus Q04400 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1 by Ni-coupled beads Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.1 brain
-
 Rattus norvegicus
-
4.6.1.1 embryo
-
 Rattus norvegicus
-
4.6.1.1 additional information AC5 highly enriched in the striatum Rattus norvegicus
-

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 AC5
-
 Rattus norvegicus
4.6.1.1 AC6
-
 Rattus norvegicus
4.6.1.1 type V adenylate cyclase
-
 Rattus norvegicus