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Literature summary extracted from

  • Hrmova, M.; Burton, R.A.; Biely, P.; Lahnstein, J.; Fincher, G.B.
    Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase (2006), Biochem. J., 399, 77-90.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.78 enzyme is rather unstable and steadily loses activity during the purification process. Addition of 0.2% bovine serum albumine stabilizes Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information Km value is 0.16 mg/ml for locust-bean galactomannan Hordeum vulgare
3.2.1.78 5.3
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man 30°C, pH 4.8 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 43000
-
 x * 43000, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Hordeum vulgare
-
 cultivar Clipper
-
3.2.1.78 Hordeum vulgare Q2I011 isoform MAN1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Hordeum vulgare
3.2.1.78 purification from seedling Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.25 seedling
-
 Hordeum vulgare
-
3.2.1.78 seedling 10 days old Hordeum vulgare
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.78 -20°C, a single freeze-thawing cycle results in loss of 80% of activity. Bovine serum albumine stabilizes Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 additional information 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 4-nitrophenyl-beta-D-xyloside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-gentiobioside, and 4-nitrophenyl-cellobioside are no substrates Hordeum vulgare ?
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-galactoside + H2O 4.6% activity compared to p-nitrophenyl-beta-D-mannoside Hordeum vulgare p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-glucoside + H2O 34.5% activity compared to p-nitrophenyl-beta-D-mannoside Hordeum vulgare p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O hydrolyses 4-nitrophenyl-beta-D-mannoside three times faster than p-nitrophenyl-beta-D-glucoside Hordeum vulgare p-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.78 galactomannan + H2O
-
 Hordeum vulgare galactosyl-mannosyl oligosaccharides products show a degree of polymerization of 2-6 ?
3.2.1.78 glucomannan + H2O
-
 Hordeum vulgare glucosyl-mannosyl oligosaccharides products show a degree of polymerization of 2-6 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Hordeum vulgare Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man
-
 ?
3.2.1.78 mannan + H2O
-
 Hordeum vulgare mannosyl oligosaccharides products show a degree of polymerization of 2-6 ?
3.2.1.78 additional information during hydrolysis of 1,4-beta-manno-oligosaccharides, transglycosylation reactions occur Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 43000, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.2.1.25 beta-D-mannosidase
-
 Hordeum vulgare
3.2.1.25 betaMANNOS1 previously identified as a beta-D-glucosidase Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 3.9
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man 30°C, pH 4.8 Hordeum vulgare
3.2.1.78 12.9
-
 galactomannan 30°C, pH 4.8 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4.8
-
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.78 Hordeum vulgare isoelectric focusing
-
 7.8