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Literature summary extracted from

  • Diaz-Benjumea, R.; Laxman, S.; Hinds, T.R.; Beavo, J.A.; Rascon, A.
    Characterization of a novel cAMP-binding, cAMP-specific cyclic nucleotide phosphodiesterase (TcrPDEB1) from Trypanosoma cruzi (2006), Biochem. J., 399, 305-314.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.53 cAMP binds to N-terminus and GAF domain, affinity of 0.0005 mM Trypanosoma cruzi
3.1.4.53 cAMP binds to N-terminus and GAF domain, affinity of 0.001 mM Trypanosoma cruzi
3.1.4.53 cGMP binds to N-terminus and GAF domain, affinity of 0.003 mM Trypanosoma cruzi
3.1.4.53 additional information enzyme does not bind cGMP Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.53 expression of TcrPDEB1 catalytic domain in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.53 dipyridamole
-
 Trypanosoma cruzi
3.1.4.53 EHNA
-
 Trypanosoma cruzi
3.1.4.53 erythro-9-(2-hydroxy-3-nonyl)adenine i.e. EHNA Trypanosoma cruzi
3.1.4.53 N-[(5S)-5-[2-(3-fluorophenyl)acetamido]-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxohexyl]prop-2-enamide
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.53 0.0112
-
 adenosine 3',5'-cyclic phosphate pH 7.5, 30°C Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.53 102500
-
 x * 102500, calculated Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.53 Trypanosoma cruzi
-
 isoform PDEB2, expression in all developmental stages
-
3.1.4.53 Trypanosoma cruzi Q49UB9
-
-
3.1.4.53 Trypanosoma cruzi Q49UB9 isoform PDEB1, expression in all developmental stages
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.53 2'-O-(N-methylanthraniloyl)adenosine-3',5'-cyclic monophosphate + H2O fluorescent cAMP analogue Trypanosoma cruzi 2'-O-(N-methylanthraniloyl)adenosine-5'-monophosphate
-
 ?
3.1.4.53 adenosine 3',5'-cyclic phosphate + H2O
-
 Trypanosoma cruzi adenosine 5'-phosphate
-
 ?
3.1.4.53 cAMP + H2O
-
 Trypanosoma cruzi 5'-AMP
-
 ?
3.1.4.53 additional information no activity with up to 0.1 mM cGMP Trypanosoma cruzi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.53 ? x * 102500, calculated Trypanosoma cruzi
3.1.4.53 More enzyme contains two GAF domains and a catalytic domain highly homologous with that of the Trypanosoma brucei family Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.1.4.53 TcrPDEB1
-
 Trypanosoma cruzi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.53 0.00113
-
 pH 7.5, 30°C Trypanosoma cruzi dipyridamole
3.1.4.53 0.0113
-
 30°C, pH 7.5 Trypanosoma cruzi dipyridamole
3.1.4.53 0.015
-
 30°C, pH 7.5 Trypanosoma cruzi dipyridamole
3.1.4.53 0.105
-
 30°C, pH 7.5 Trypanosoma cruzi N-[(5S)-5-[2-(3-fluorophenyl)acetamido]-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxohexyl]prop-2-enamide
3.1.4.53 0.128
-
 30°C, pH 7.5 Trypanosoma cruzi EHNA