EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.2.26 | diagnostics | the enzyme is a diagnostic marker of an enhanced proteoglycan biosynthesis | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.26 | soluble mutant enzymes of human XT-I with deletions at the N-terminal domain are expressed in insect cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.26 | C276A | mutation results in a nearly inactive enzyme | Homo sapiens |
2.4.2.26 | DELTA1-184 | KM-value for bikunin is identical to wild-type value, Vmax is 1.2fold higher than wild-type value | Homo sapiens |
2.4.2.26 | DELTA1-213 | KM-value for bikunin is 1.6fold higher than wild-type value, Vmax is 1.1fold higher than wild-type value | Homo sapiens |
2.4.2.26 | DELTA1-260 | KM-value for bikunin is 1.6fold higher than wild-type value, Vmax is identical to wild-type value | Homo sapiens |
2.4.2.26 | DELTA1-266 | KM-value for bikunin is 5.6fold higher than wild-type value, Vmax is 1.6fold lower than wild-type value | Homo sapiens |
2.4.2.26 | DELTA1-272 | KM-value for bikunin is 6fold higher than wild-type value, Vmax is 1.3fold lower than wild-type value | Homo sapiens |
2.4.2.26 | DELTA1-273 | more than 98% of activity | Homo sapiens |
2.4.2.26 | DELTA261-272 | inactive mutant enzyme | Homo sapiens |
2.4.2.26 | DELTA721-726 | inactive mutant enzyme | Homo sapiens |
2.4.2.26 | E263A | KM-value for bikunin is 1.4fold higher than wild-type value, Vmax is 2fold higher than wild-type value | Homo sapiens |
2.4.2.26 | K262A | KM-value for bikunin is 1.2fold higher than wild-type value, Vmax is 1.7fold higher than wild-type value | Homo sapiens |
2.4.2.26 | K272A | KM-value for bikunin is 1.4fold higher than wild-type value, Vmax is 1.7fold higher than wild-type value | Homo sapiens |
2.4.2.26 | additional information | truncation of 266, 272 and 273 amino acids in the N-terminal region results in a 70, 90 and above 98% loss in catalytic activity. Deletion of the single 12 amino acid motif G261KEAISALSRAK272 leads to a loss-of-function xylosyltransferase I mutant. Heparin binding is slightly altered in mutants lacking 289 or 568 amino acids, but deletion of the potential heparin-binding motif P721KKVFKI727 does not lead to a loss of heparin binding capacity | Homo sapiens |
2.4.2.26 | R270A | KM-value for bikunin is 1.2fold higher than wild-type value, Vmax is 1.4fold higher than wild-type value | Homo sapiens |
2.4.2.26 | S266A | KM-value for bikunin is 1.4fold higher than wild-type value, Vmax is 1.3fold higher than wild-type value | Homo sapiens |
2.4.2.26 | S269A | KM-value for bikunin is 1.3fold higher than wild-type value, Vmax is 1.1fold higher than wild-type value | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.26 | 0.0009 | - |
bikunin | 37°C, pH 6.5 | Homo sapiens | |
2.4.2.26 | 0.0009 | - |
bikunin | 37°C, pH 6.5, mutant enzyme DELTA1-184 | Homo sapiens | |
2.4.2.26 | 0.0011 | - |
bikunin | 37°C, pH 6.5, mutant enzyme K262A | Homo sapiens | |
2.4.2.26 | 0.0011 | - |
bikunin | 37°C, pH 6.5, mutant enzyme R270A | Homo sapiens | |
2.4.2.26 | 0.0012 | - |
bikunin | 37°C, pH 6.5, mutant enzyme S269A | Homo sapiens | |
2.4.2.26 | 0.0013 | - |
bikunin | 37°C, pH 6.5, mutant enzyme E263A | Homo sapiens | |
2.4.2.26 | 0.0013 | - |
bikunin | 37°C, pH 6.5, mutant enzyme K272A | Homo sapiens | |
2.4.2.26 | 0.0013 | - |
bikunin | 37°C, pH 6.5, mutant enzyme S266A | Homo sapiens | |
2.4.2.26 | 0.0014 | - |
bikunin | 37°C, pH 6.5, mutant enzyme DELTA1-213 | Homo sapiens | |
2.4.2.26 | 0.0014 | - |
bikunin | 37°C, pH 6.5, mutant enzyme DELTA1-260 | Homo sapiens | |
2.4.2.26 | 0.005 | - |
bikunin | 37°C, pH 6.5, mutant enzyme DELTA1-266 | Homo sapiens | |
2.4.2.26 | 0.0054 | - |
bikunin | 37°C, pH 6.5, mutant enzyme DELTA1-272 | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.26 | UDP-alpha-D-xylose + [protein]-L-serine | Homo sapiens | initiates the biosynthesis of the glycosaminoglycan linkage region | UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.26 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.26 | UDP-alpha-D-xylose + [protein]-L-serine | initiates the biosynthesis of the glycosaminoglycan linkage region | Homo sapiens | UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine | - |
? | |
2.4.2.26 | UDP-D-xylose + bikunin | - |
Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.26 | xylosyltransferase I | - |
Homo sapiens |