Any feedback?
Literature summary extracted from
- Purification and characterization of a heat-stable esterase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. (1988), Biochem. J., 250, 453-458.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | hanging-drop vapour diffusion method | Sulfolobus acidocaldarius |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.1 | eserine | 10 mM, 19% inhibition | Sulfolobus acidocaldarius |  |
| 3.1.1.1 | paraoxon | 0.01 mM, complete inhibition | Sulfolobus acidocaldarius | |
| 3.1.1.1 | phenylmethanesulfonyl fluoride | 0.1 mM, complete inhibition | Sulfolobus acidocaldarius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 0.01036 | - |
4-nitrophenyl hexanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
| 3.1.1.1 | 0.0215 | - |
4-nitrophenyl acetate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
| 3.1.1.1 | 0.0338 | - |
4-nitrophenyl propionate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
| 3.1.1.1 | 0.0606 | - |
4-nitrophenyl octanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
| 3.1.1.1 | 0.1287 | - |
4-nitrophenyl butanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
| 3.1.1.1 | 0.1517 | - |
4-nitrophenyl pentanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 32000 | - |
4 * 32000, SDS-PAGE | Sulfolobus acidocaldarius |
| 3.1.1.1 | 117000 | - |
meniscus depletion sedimentation equilibrium in the analytical ultracentrifuge | Sulfolobus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Sulfolobus acidocaldarius | Q7M529 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | - |
Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 158 | - |
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | 8.8% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl butanoate + H2O | 83% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + butanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl decanoate + H2O | 1.8% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl dodecanoate + H2O | 0.8% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + dodecanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl hexanoate + H2O | 75.4% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl octanoate + H2O | 11.5% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl pentanoate + H2O | - |
Sulfolobus acidocaldarius | 4-nitrophenol + pentanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl propionate + H2O | 18% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + propionate | - |
? | |
| 3.1.1.1 | ethyl valerate + H2O | - |
Sulfolobus acidocaldarius | ethanol + valeric acid | - |
? | |
| 3.1.1.1 | hexyl valerate + H2O | - |
Sulfolobus acidocaldarius | hexanol + valeric acid | - |
? | |
| 3.1.1.1 | additional information | no hydrolysis of triolein and ethyl acetate | Sulfolobus acidocaldarius | ? | - |
? | |
| 3.1.1.1 | pentyl acetate + H2O | - |
Sulfolobus acidocaldarius | pentanol + acetate | - |
? | |
| 3.1.1.1 | pentyl valerate + H2O | not hydrolysed in the absence of Triton X-100 | Sulfolobus acidocaldarius | pentanol + valeric acid | - |
? | |
| 3.1.1.1 | triacetin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? | |
| 3.1.1.1 | tributyrin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | tetramer | 4 * 32000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 80 | - |
1 h, stable | Sulfolobus acidocaldarius |
| 3.1.1.1 | 90 | - |
1 h, 8% loss of activity | Sulfolobus acidocaldarius |
| 3.1.1.1 | 100 | - |
1 h, 50% loss of activity | Sulfolobus acidocaldarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7.5 | 8.5 | - |
Sulfolobus acidocaldarius |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.1 | Sulfolobus acidocaldarius | isoelectric focusing | - |
4.9 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
