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Literature summary extracted from

  • Amouri, B.; Gargouri, A.
    Characterization of a novel beta-glucosidase from a Stachybotrys strain (2006), Biochem. Eng. J., 32, 191-197.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 additional information no inhibition by SDS and 2-mercaptoethanol as long as the enzyme is not preheated Stachybotrys sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.27
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.8, 50°C Stachybotrys sp.
3.2.1.21 2.22
-
 cellobiose pH 4.8, 50°C Stachybotrys sp.
3.2.1.21 6.2
-
 carboxymethylcellulose pH 4.8, 50°C Stachybotrys sp.
3.2.1.21 37.14
-
 Salicin pH 4.8, 50°C Stachybotrys sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Stachybotrys sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 85000
-
 2 * 85000, SDS-PAGE Stachybotrys sp.
3.2.1.21 170000
-
 about, gel filtration Stachybotrys sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellobiose + H2O Stachybotrys sp.
-
 2 beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Stachybotrys sp.
-
 mutant strain A19 is isolated from the cellulolytic fungus strain N1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native enzyme from culture supernatant to homogeneity by anion exchange chromatography and gel filtration Stachybotrys sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information the fungus is fed-batch cultivated on cellulose Stachybotrys sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Stachybotrys sp. 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Stachybotrys sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 carboxymethylcellulose + H2O
-
 Stachybotrys sp. beta-D-glucose + 2-(hydroxymethyl)phenol
-
 ?
3.2.1.21 cellobiose + H2O
-
 Stachybotrys sp. 2 beta-D-glucose
-
 ?
3.2.1.21 salicin + H2O low activity Stachybotrys sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 dimer 2 * 85000, SDS-PAGE Stachybotrys sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 aryl beta-glucosidase
-
 Stachybotrys sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Stachybotrys sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21
-
 100 50% of maximal activity at 0°C, 30°C, and 100°C Stachybotrys sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 70
-
 inactivation of the purified enzyme after 15 min Stachybotrys sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Stachybotrys sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3 9 50% of maximal activity at pH 3.0 and pH 9.0 Stachybotrys sp.