Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, J.; Xia, W.
    Purification and characterization of a bifunctional enzyme with chitosanase and cellulase activity from commercial cellulase (2006), Biochem. Eng. J., 30, 82-87.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.132 Co2+ 2.5 mM, 16% increase in activity Trichoderma viride
3.2.1.132 Mn2+ 3 mM, 18% increase in activity Trichoderma viride

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ag+
-
 Trichoderma viride
3.2.1.4 Hg2+
-
 Trichoderma viride
3.2.1.132 Ag+ 1 mM, 20% loss of activity Trichoderma viride
3.2.1.132 Hg2+ 1 mM, 80% loss of activity Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information Km-value is 0.1 mg/ml glucose for hydrolysis of carboxymethyl cellulose Trichoderma viride
3.2.1.132 additional information
-
 chitosan Km value is 10 mg/ml Trichoderma viride

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 64000
-
 gel filtration Trichoderma viride
3.2.1.4 66000
-
 1 * 66000, SDS-PAGE Trichoderma viride
3.2.1.132 64000
-
 gel filtration Trichoderma viride
3.2.1.132 66000
-
 1 * 66000, SDS-PAGE Trichoderma viride

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma viride
-
-
-
3.2.1.132 Trichoderma viride
-
 commercial preparation of cellulase, isolation of a bifunctional chitosanase/cellulase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Trichoderma viride
3.2.1.132 isolation of a bifunctional chitosanase/cellulase from commercial prepapration of cellulase Trichoderma viride

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 0.258
-
-
 Trichoderma viride
3.2.1.132 0.402
-
 pH 5.2, 60°C Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.4 additional information the hydrolase exhibited chitosanase activity and cellulase activity Trichoderma viride ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Trichoderma viride glucosamine oligomer + N-acetyl-glucosamine oligomer hydrolysates are oligomers with one to four glucosamine residues and some oligomers with longer chain length ?
3.2.1.132 additional information enzyme cleaves both glucosamine-glucosamine and N-acetylglucosamine-glucosamine linkages of chitosan. Enzyme additionally has cellulase activity for carboxymethylcellulose Trichoderma viride ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 66000, SDS-PAGE Trichoderma viride
3.2.1.132 monomer 1 * 66000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Trichoderma viride
3.2.1.132 60
-
-
 Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 30 70 30 min to 2 h, stable Trichoderma viride
3.2.1.132 30 70 stable for 2 h Trichoderma viride
3.2.1.132 80
-
 15 min, almost complete loss of activity Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.2
-
 hydrolysis of carboxymethyl cellulose Trichoderma viride
3.2.1.132 5.2
-
 chitosan hydrolysis Trichoderma viride

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3 7 30°C, 24 h, stable Trichoderma viride
3.2.1.132 3 7 30°C, stable for at least 24 h Trichoderma viride