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Literature summary extracted from

  • Wang, T.; Lee, W.
    Production of 2-keto-3-deoxy-D-glycero-D-galacto-nonopyranulosonic acid (KDN) using fusion protein of N-acetyl-D-neuraminic acid aldolase (2006), Biochem. Eng. J., 29, 75-80.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.3 expressed in Escherichia coli strain BL 21 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 2.8
-
 N-acetyl-neuraminic acid in 0.1 M phosphate buffer, pH 7 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 58890
-
 GST-tagged enzyme, calculated from amino acid sequence Escherichia coli
4.1.3.3 60000
-
 GST-tagged enzyme, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.3 glutathione-Uniflow Resin column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.3 0.14
-
 crude cell extract, at 37°C Escherichia coli
4.1.3.3 14.6
-
 after purification, at 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 D-mannose + pyruvic acid
-
 Escherichia coli 2-keto-3-deoxy-D-glycero-D-galacto-nonopyranulosonic acid
-
 ?
4.1.3.3 N-acetyl-neuraminic acid
-
 Escherichia coli N-acetyl-D-mannosamine + pyruvate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.3.3 N-acetyl-d-neuraminic acid aldolase
-
 Escherichia coli
4.1.3.3 Neu5Ac aldolase
-
 Escherichia coli