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Literature summary extracted from

  • Beluzic, R.; Cuk, M.; Pavkov, T.; Baric, I.; Vugrek, O.
    S-Adenosylhomocysteine hydrolase (AdoHcyase) deficiency: enzymatic capabilities of human AdoHcyase are highly effected by changes to codon 89 and its surrounding residues (2008), Biochem. Biophys. Res. Commun., 368, 30-36.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.1 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.13.2.1 A89V more than 70% decrease in enzyme activity and decrease in unfolding temperature by 5.5°C. Subunits assemble similarly to wild-type, but electrophoretic mobility is faster than wild-type Homo sapiens
3.13.2.1 A89V/T84S restores most of the catalytic activity of mutant A89V due to loss of sterical incompatibility of V89 and T84 Homo sapiens
3.13.2.1 T84L inactivation Homo sapiens
3.13.2.1 T84Q inactivation Homo sapiens
3.13.2.1 T84S activity similar to wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.0144
-
 S-adenosyl-DL-homocysteine mutant T84S Homo sapiens
3.13.2.1 0.0146
-
 S-adenosyl-DL-homocysteine mutant A89V Homo sapiens
3.13.2.1 0.0151
-
 S-adenosyl-DL-homocysteine wild-type Homo sapiens
3.13.2.1 0.017
-
 S-adenosyl-DL-homocysteine mutant A89V/T84S Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 260000
-
 gel filtration, wild-type Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 0.17
-
 mutant A89V, hydrolysis of S-adenosyl-DL-homocysteine Homo sapiens
3.13.2.1 0.4
-
 mutant A89V, synthesis of S-adenosyl-DL-homocysteine Homo sapiens
3.13.2.1 0.59
-
 mutant A89V/T84S, hydrolysis of S-adenosyl-DL-homocysteine Homo sapiens
3.13.2.1 0.74
-
 mutant T84S, hydrolysis of S-adenosyl-DL-homocysteine Homo sapiens
3.13.2.1 0.748
-
 wild-type, hydrolysis of S-adenosyl-DL-homocysteine Homo sapiens
3.13.2.1 1.23
-
 wild-type, synthesis of S-adenosyl-DL-homocysteine Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -20°C, wild-type enzyme is robust and retains activity for at least one year with several freeze-thaw cycles. Mutant A89V, stable for about 1 month Homo sapiens
3.13.2.1 4°C, mutant A89V, half-life of 1-2 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 DL-homocysteine + adenosine
-
 Homo sapiens S-adenosyl-DL-homocysteine + H2O
-
 r
3.13.2.1 S-adenosyl-DL-homocysteine + H2O
-
 Homo sapiens DL-homocysteine + adenosine
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.2.1 NAD+ wild-type, about 81% of cofactor bound as NAD+, mutant A89V, about 70.5% of cofactor bound as NAD+ Homo sapiens
3.13.2.1 NADH wild-type, about 81% of cofactor bound as NAD+, mutant A89V, about 70.5% of cofactor bound as NAD+ Homo sapiens