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Literature summary extracted from

  • Kang, H.K.; Cha, H.; Yang, T.J.; Park, J.T.; Lee, S.; Kim, Y.W.; Auh, J.H.; Okada, Y.; Kim, J.W.; Cha, J.; Kim, C.H.; Park, K.H.
    Enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction using TreX, a Sulfolobus solfataricus P2 debranching enzyme (2008), Biochem. Biophys. Res. Commun., 366, 98-103.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.25 synthesis the enzyme is useful in the enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction Saccharolobus solfataricus
3.2.1.68 analysis Di-O-a-maltosyl-beta-cyclodextrin is fromed via a transglycosylation reaction using TreX, a Sulfolobus solfataricus P2 debranching enzyme, isoamylase is used to examine the structure of the product and to produce branched cyclodextrins via reverse reaction Pseudomonas amyloderamosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expression of His-tagged enzyme in Escherichia coli strain MC1061 Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 beta-cyclodextrin a mixed-type inhibitor Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 1.591
-
 maltosyl-beta-cyclodextrin pH 5.5, 75°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Saccharolobus solfataricus
-
 gene treX
-
2.4.1.25 Saccharolobus solfataricus P2
-
 gene treX
-
3.2.1.68 Pseudomonas amyloderamosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 recombinant His-tagged enzyme from Escherichia coli strain MC1061 by nickel affinity chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltose + beta-cyclodextrin condensation reaction mechanism, overview Saccharolobus solfataricus 6-O-alpha-maltosyl-beta-cyclodextrin mass spectrometric product identification ?
2.4.1.25 maltose + beta-cyclodextrin condensation reaction mechanism, overview Saccharolobus solfataricus P2 6-O-alpha-maltosyl-beta-cyclodextrin mass spectrometric product identification ?
2.4.1.25 maltose + maltosyl-beta-cyclodextrin
-
 Saccharolobus solfataricus ?
-
 ?
2.4.1.25 maltose + maltosyl-beta-cyclodextrin
-
 Saccharolobus solfataricus P2 ?
-
 ?
2.4.1.25 additional information synthesis of di-O-alpha-maltosyl-beta-cyclodextrin from 6-O-alpha-maltosyl-beta-cyclodextrin via a transglycosylation reaction, TreX transfers the maltosyl residue of a G2-beta-cyclodextrin to another molecule of G2-beta-cyclodextrin by forming an alpha-1,6-glucosidic linkage. TreX shows specificity for a branched glucosyl chain bigger than DP2 and no activity toward glucosyl-beta-cyclodextrin, transglycosylation reaction mechanism, overview Saccharolobus solfataricus ?
-
 ?
2.4.1.25 additional information synthesis of di-O-alpha-maltosyl-beta-cyclodextrin from 6-O-alpha-maltosyl-beta-cyclodextrin via a transglycosylation reaction, TreX transfers the maltosyl residue of a G2-beta-cyclodextrin to another molecule of G2-beta-cyclodextrin by forming an alpha-1,6-glucosidic linkage. TreX shows specificity for a branched glucosyl chain bigger than DP2 and no activity toward glucosyl-beta-cyclodextrin, transglycosylation reaction mechanism, overview Saccharolobus solfataricus P2 ?
-
 ?
3.2.1.68 di-O-alpha-maltosyl-beta-cyclodextrin + H2O
-
 Pseudomonas amyloderamosa maltose + beta-cyclodextrin
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 debranching enzyme
-
 Saccharolobus solfataricus
2.4.1.25 TreX
-
 Saccharolobus solfataricus
3.2.1.68 glycogen alpha-1,6-glucanohydrolase
-
 Pseudomonas amyloderamosa
3.2.1.68 isoamylase
-
 Pseudomonas amyloderamosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 70 75 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 0.0113
-
 maltosyl-beta-cyclodextrin pH 5.5, 75°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 5.5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.25 0.0556
-
 beta-cyclodextrin pH 5.5, 75°C, recombinant His-tagged enzyme Saccharolobus solfataricus