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Literature summary extracted from

  • Li, S.; Li, B.; Fei, Y.; Jiang, D.; Sheng, Y.; Sun, Y.; Zhang, J.
    Exon grafting yields a "two active-site" lysozyme (2007), Biochem. Biophys. Res. Commun., 358, 997-1001.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.17 expression in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.17 additional information a strategy to engineer an additional active site for human lysozyme: grafting the entire human lysozyme exon 2, which encodes the catalytically competent domain, into the gene at a position corresponding to an exposed loop region in the translated protein. Exon 2 grafting creates a novel lysozyme with twice the activity of the wild type enzyme, equal activity came from each of the two active sites. The thermal stability and pH-stability of wild-type and two-active site lysozyme is similar Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.17
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.17 no significant loss of the activity after 6 months of frozen storage for both wild-type and two-active site lysozyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 peptidoglycan + H2O lytic activity against Micrococcus lysodeikticus Homo sapiens ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.17 5.9
-
 wild-type enzyme, lytic activity against Micrococcus lysodeikticus Homo sapiens
3.2.1.17 6.5
-
 two-active site lysozyme, lytic activity against Micrococcus lysodeikticus Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.17 5.4 7.2 wild-type and two-active site lysozyme both retain over 80% activity as determined by assaying the lysis of Micrococcus lysodeikticus cells over a pH range of 5.3 to 7.2 Homo sapiens